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品　　牌：WALZ

产品型号：ZQ-WALZ001 (IMAGING-PAM )

制造厂商：上海泽泉科∩技有限公司

所在地区：上海

M系列调制叶绿素荧光成像系统IMAGING-PAM

简便、快速、可靠、对样品无干扰
代表了调制荧光技术的未来发展方向！
Schreiber教授因发明PAM系列调制叶绿素【荧光仪而获得首届国际光合作用协会（ISPR）创新奖

叶片被激光穿孔后NPQ信号的动态传递过程

传统的光纤型调制荧光仪（如PAM-2100、MINI-PAM等）只能测量叶片上一个点的光合活性。利用一个点的数据代表一个叶片，利用一个叶片代表一个植株，进ζ而代表一个群体（如森林、大田作物等），这种方法①的误差是比较大的。

从1980年代末期开始，科研人员就期望能通过成像型荧光仪来测量全〖叶片光合活性→，并进行了不懈的尝试，但受技术上的限制，所设计的仪器无法商品化或商品化了但得不到大家认可。其♀中一个很重要的原因就是能够发出饱和脉冲水平强光的二极管（LED）尚未面世。要利用调制荧光技术测量全叶片水平的光合作用，首先要保证叶片上任何Ψ一点所接受到的光强必须是完全相同的。调制荧光技术要求光源必须能发※出很强的饱和脉冲光。卤素灯能发出很强的光，但其光↙场非常不均匀，根本不能用于成像！装在一个平面上的LED阵列发出的光很均匀，但在2000年前，能发出超▓强光合有效辐射（PAR）的LED根本没有】面世！

2000年，能发出超强PAR的蓝光LED面世。2001年，全球最权威的调制荧光仪制造商德国WALZ公司设计制造了真正的全球第一台多功能调「制荧光成像系统IMAGING-PAM。IMAGING-PAM采用超强发光LED作为光源，保证叶片表明受光均¤匀且光强足够强；IMAGING-PAM采用CCD作为检测器，能检测叶片上每个像＠ 素的光合作用；IMAGING-PAM秉承了WALZ公司PAM系列荧光仪的一贯优点，功能强大，测量参数多，操作极其简单，一面世就受到全球植物学家的青睐，迅速占领全球市场。

2005年，WALZ又推出了M系列IMAGING-PAM，一个主机■可以连接不同的探头（MICROSCOPY-，MICRO-，MINI-和MAXI-探头），分别在130×150 um、3.5×4.5 mm、24×32 mm或10×13 cm的面积上测量荧光成像。现在，只需一个主机连接不同的探头，即可满足从单细胞到全叶片，从分子生物学到生态学研究的全面需要。

M系列IMAGING-PAM不同版本的比较

MAXI-版	MINI-版	MICRO-版	MICROSCOPY-版
成像面积10×13 cm	成像面积24×32 mm	成像面积3.5×4.5 mm	成像面积130×150 um
放大1.5倍	放大6倍	放大45倍	放大130-1300倍

功 能
* 一个主机连接不同的探头可满□　足从单细胞到全叶片、从分子生物学到生态学的不同需求
* 全叶片光合作用分析（荧光成像），可测荧光诱导曲线◆并进行淬灭分析
* 可测快速光响应曲线（120 s内完成，比光合放氧和气体→交换等技术快得多）
* 叶片光合作用的横向异质性检测
* 完全相同的条件下同时测量多●个样品（植物、地衣、苔藓、微藻等）
* 遗传育种、突变※株筛选的强大工具
* 不同的测量面积，不同的分辨率
* 可利◥用多孔板（如96孔板）做多个微藻样品的同时成像
* 胁迫损伤的早期检测
* 不连接显微镜即可测量绿色荧光蛋白（GFP）荧光
* 可测量叶片吸光系数
应用范围

* 环境科学 * 水生卐生物学 * 海洋与湖沼学 * 生态毒理学 * 园艺学 * 农业科学 * 林学

* 环境科学 * 水『生生物学 * 海洋与湖沼学 * 生态毒理学 * 园艺学 * 农业科学 * 林学

DCMU在叶片中的渗透过程

测量参数


* 以上所有参数均可成像
* 吸光系数Abs和新参数qL、Y(NPQ)和Y(NO)的成像是IMAGING-PAM独有的
* 生态毒理学研究中，选▓一个参考点，可以直接求出其它处理（如农药）的受抑制程度Inh.



各种荧光参数的成像是将0.0（黑色）至1.0（紫色）的数值转换成颜色来显示的。

M系列IMAGING-PAM不同版本介绍

MAXI-版 大探头，成像面积10×13 cm 调制荧光成像系统的MAXI-探头利用300 W的LED阵列，可以在10×13 cm的面积上提供均为的调制测量光、光化光和饱和脉冲光。该探头的支架上配备特制护眼遮光罩，可以在保护眼睛的同时观测到红色荧光的●变化。 WALZ提供两种数码相机CCD供选择。用户若需要高清晰度，推荐选择IMAG-MAX/K[2/3” chip, 1392×1040象素， 4象素组合（binning）技术]。标ζ　准应用可选择IMAG-MAX/K2（1/2”, 640×480象素），可与IMAG-MAX/K2Z物镜（F1.0/f=8-48mm）结合使用。

由于MINI-探头的※便携式设计，使其特别适合野外应用。由于MINI-探头的成像面积仅为MAXI-探头的1/16，因而前者发出的最大光强更大，但耗电却小得多。MINI-探头可以安装在光合仪GFS-3000的叶室3010-S上，同步测量全▆叶片气体交换和荧光成像，并且其光源可由GFS-3000控制，达到真正■的同步测量。
MINI-探头采用1/3”数码相机CCD（640×480象素）和F1.2/f=12mm物镜。其设计目的为测量固定距离下的荧光成像。

与光合仪GFS-3000连用	长时间测量可装在三角架上①	Fm	qN

Micro-版 微探头，成像面积3.5×4.5 mm 调制荧光成像系统的MICRO-探头是一个极便携的探头，采用整合式Cosmicar-Pentax CCTV物镜（F1.4/f=16mm），直接安装在数码CCD（1/3” chip, 640×480像素）上。 MICRO-探头只配备一个Luxeon LED（蓝光，450 nm）和一个特制双色分光镜，类似于落射荧光显微镜。 尽管成像面积只有3.5×4.5 mm，但45倍的放大率却允许对叶片荧光成像的异质性分析达到支脉（minor veins）级。同时还可提供一个特制版本用于测量GFP的成像。

MICRO-探头还可安装在标准版IMAGING-PAM（2001年设计）主机上。该探头◎提供X-Y轴可调的样品台。其设计目的为测量固定距离下的荧光成像。

Fo	GFP成像	微探头直接安装在CCD上

Microscopy-版 显微探头，成像面积130×150 um 必须与特制落射荧光显微镜（Hund或Zeiss）结合使用，该显微镜可以提供激〗发光并检测荧光 IMAG-MAX/K（数码相机CCD）[1392×1040象素，4象素组合（binning）技术]可以提供高灵敏度。 探头标准配置＠是一个超强Luxeon LED（450-480 nm），用于提供测量光、光化光和饱和脉冲。目前已可提供RGB探头（红-绿-蓝-白LED光源），它采用了PHYTO-PAM技术，可以显微镜下自动对蓝藻、绿藻、硅/甲藻、红藻进行分类并测量光合作用。

分类，红色为硅█藻，绿色为丝状绿藻	光合，Fv/Fm活性，可区分细胞不同部位的活性

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