详细说明
Anti-Human Interleukin-2 Receptor Alpha (IL-2 Rα)Purified No Carrier Protein(IL2RA, IDDM10, CD25, IL2R, TCGFR, P55, Tac)Monoclonal AntibodyProd. No.: I-167 Clone: 22722 Isotype: Mouse IgG1 Pkg. Size: 500 μg Storage: -20°C to -70°C
Anti-Human Interleukin-2 Receptor Alpha (IL-2 Rα)
Purified No Carrier Protein
(IL2RA, IDDM10, CD25, IL2R, TCGFR, P55, Tac)
Monoclonal Antibody
Prod. No.: I-167
Clone: 22722
Isotype: Mouse IgG1
Pkg. Size: 500 µg
Storage: -20°C to -70°C
Description
Specificity:
Mouse Anti-Human Interleukin-2 Receptor alpha (IL-2Rα) (Clone 22722) recognizes an epitope on Human IL-2Rα. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background:
IL-2 receptor alpha chain (IL-2Ralpha) is a component of high-affinity IL-2 receptors and thus is a key regulator of lymphocyte proliferation.1 IL-2R alpha expression occurs at specific stages of early T and B lymphocyte development and is induced upon activation of mature lymphocytes. IL-2R alpha is essential for regulation of both the size and content of the peripheral lymphoid compartment, probably by influencing the balance between clonal expansion and cell death following lymphocyte activation.2 IL-2Ralpha is also important in the formation of high-affinity binding sites, and the inducibility of the alpha gene contributes to the highly regulated and transient display of high-affinity IL-2R.3
Host Species
Mouse
Immunogen
Purified Recombinant Human sIL-2R (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbeccos phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, IHC (NBF/Par.), N, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). The detection sensitivity of this indirect ELISA for Human IL-2Rα is approximately 0.3 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 2-15 µg/ml. The recommended secondary antibody for IHC is Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114). For chromogenic detection with high signal and low background use 3, 3-Diaminobenzidine Tetrahydrochloride (DAB) Substrate Kit (Leinco Prod. No.: D100) or Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107).
Neutralization: This antibody is useful for neutralization of Human IL-2R α bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human IL-2R α (at 1 ng/ml) is 0.5-1.0 µg/ml.
Western Blotting: To detect Human IL-2Rα this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Mouse IgG (H&L)-Horseradish Peroxidase (Leinco Prod. No.: M114) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Human IL-2Rα is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
References
1. Warren J. Leonard et al. (2002) The EMBO Journal 21: 3051
2. FW Alt et al. (1995) Immnnity 3: 521
3. Greene WC et al. (1990) J Invest Dermatol. 94: 27S
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Optional Metal Enhanced DAB Substrate Kit (Leinco Prod. No.: K107)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
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