详细说明
Neurofilament M Phospho (pS614/619) Antibody Rabbit Monoclonal Antibody Cat.# 2275-1
Neurofilament M Phospho （pS614/619） Antibody
Rabbit Monoclonal Antibody | | Cat.# 2275-1 |
Description
Neuofilaments （NF） are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton （1）. NFs are composed of three major proteins; NF-H, NF-L ａnd NF-M ａnd the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein （200 kDa）, NF-M the medium （160 kDa） ａnd NF-L the light protein （68kDa） （2）. Several phsopholyation sites have been discovered on NF-M, including serine 614 ａnd 619. However, their specific implication on NF-M function has not been identified. | |
Recommended Applications
WB, IP | | | | Applications ａnd Recommended Dilution Factors | | | | WB | IHC | ICC | FC | IP |
| | 1:500 | 1:100 - 250 | | | 1:30 | |
| Species Reactivity* | | | | Human | Mouse | Rat |
| | Positive | Negative | Negative | *Cross reactivity determined by western blot only. |
|
| Product Data |
 |  | | A. Western blot analysis on SH-SY5Y cell lysates using anti- NF-M （pS614/619） RabMAb （cat.# 2275-1）, 1:500 dilution. | B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-NF-M RabMAb （cat# 2275-1）. | |
Specificity
A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human NF-M was used as an immunogen. |
Storage Buffer & Conditions
50 mM Tris-Glycine （pH 7.4）, 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide ａnd 0.05% BSA. |
Alternative Names
NEFM, NEF3, NFM, Neurofilament medium polypeptide, Neurofilament triplet M protein;160 kDa neurofilament protein;Neurofilament 3 |
Description References
1. Nixon, R.A., ａnd Shea, T. B. （1992） Cell Motil. Cytoskeleton 22, 81-91
2. Julien, JP., Meyer, D., Flavell, D., Hurst, J., ａnd Grosveld, F. （1986） Brain Res. 387, 243-250
3. Kim SK et al. J Neurosci Methods Dec 2006.
4. Lin H et al. Hum Mol Genet 14（23）:3643-59, 2005. |
