详细说明
FYB Antibody Rabbit Monoclonal Antibody Cat.# 2361-1 Clone ID: EP2546YSwiss Prot: O15117 Mol Weight: 120 kDaSize: 100ul
Description
FYN-binding protein (FYB) is an adapter protein for Fyn and SLP-76 signaling cascades in T cells. A hematopoietic protein, FYB associates with p59fyn and becomes tyrosine-phosphorylated in response to receptor ligation. FYB has been associated to modulation of IL-2 expression in T cells and platelet activation (3). Fyn (p59Fyn) is a 59 kDa member of the Src family of tyrosine kinases. The carboxy terminus of Fyn shares extensive amino acid sequence homology with Src, but is very different within the amino-terminal 81 amino acid residues. The Fyn protein is synthesized and N-myristoylated on cytosolic polysomes and then rapidly targeted to the plasma membrane, where it is palmitoylated. Fyns biological functions are diverse and include signaling via the T cell receptor, regulation of brain function and adhesion mediated signaling (2, 3). | |
Recommended Applications
WB, IHC, ICC, FC | | | | Applications and Recommended Dilution Factors | | | | WB | IHC | ICC | FC | IP |
| | 1:5000 - 10,000 | 1:250 - 500 | 1:100 - 250 | 1:60 | | |
| Species Reactivity* | | | | Human | Mouse | Rat |
| | Positive | Negative | Negative | *Cross reactivity determined by western blot only. | |
| Product Data |
 |  | | B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-FYB RabMAb (cat# 2361-1) | A. Western blot analysis on TF-1 lysate using anti-FYB RabMAb (cat# 2361-1), 1:10,000 dilution. | |
Specificity
A synthetic peptide corresponding to residues on the N-terminus of human FYB was used as an immunogen. |
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Alternative Names
FYB, SLAP130, FYN-binding protein, FYN-T-binding protein;FYB-120/130;p120/p130;SLP-76-associated phosphoprotein;SLAP-130;Adhesion and degranulation promoting adaptor protein |
Description References
1. Renzoni D.A., et al; Biochemistry 35:15646-1565, 1996.
2. Mustelin T, et al., Eur J Immunol. 1992 May;22(5):1173-8.
3. Silva AJ, et al. PNAS USA, 94(14):7493-7498, 1997. |
