详细说明
Ferritin Antibody Rabbit Monoclonal Antibody Cat.# 2443-1 Clone ID: EPR3004YSwiss Prot: P02794 Mol Weight: 21/19 kDaSize: 100ul
Description
Ferritin is important in iron homeostasis. It is the main iron-storage protein, composed of two partially homologous subunits, heavy and light chains. Ferritin molecules in cells containing high levels of iron tend to be rich in light chains, and may have a long-term storage function, whereas heavy-rich ferritins are more active in iron metabolism (1-2). Mutations in the ferritin gene cause the hereditary hyperferritinemia-cataract syndrome and neuroferritinopathy, associated with inflammation. Elevated levels of ferritin are reported as characteristic of adult-onset Stills disease and hemophagocytic syndrome, also associated with inflammation (3). Microfilaments and increased levels of exogenous ferritin are excreted directly into the bile by way of a second microfilament-independent, chloroquine-insensitive pathway, supporting a possible physiological mechanism for the release of ferritin from the liver (4). | |
Recommended Applications
WB, ICC | | | | Applications and Recommended Dilution Factors | | | | WB | IHC | ICC | FC | IP |
| | 1:1000 - 2000 | | 1:100 | | | |
| Species Reactivity* | | | | Human | Mouse | Rat |
| | Positive | Positive | Positive | *Cross reactivity determined by western blot only. | |
| Product Data |
 | | | A. Western blot analysis on (A) Jurkat, (B) SH-SY-5Y and (C) human brain lysates using anti-Ferritin RabMAb (cat. # 2443-1), dilution 1:2000. | | |
Specificity
A synthetic peptide corresponding to residues in human Ferritin was used as an immunogen. This antibody recognizes both, Ferritin heavy and light chains. |
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Alternative Names
FTH1, FTH, FTHL6, Ferritin heavy chain, Cell proliferation-inducing gene 15 protein |
Description References
1. Boyd D, et al. Proc Natl Acad Sci U S A. 81(15):4751-5,1984
2. Lawson DM, et al. Nature. 349(6309):541-4, 1991
3. Zandman-Goddard G, et al. Autoimmunity Reviews
6(7):457-463, 2007
4. Ramm G A, et al. Hepatology 19(2): 504-513, 2005 |
