详细说明
Fc gamma RIIb (C-term) Antibody Rabbit Polyclonal Antibody Cat.# 1723-1 Clone ID: PolyclonalSwiss Prot: P31994 Mol Weight: 40-44 kDaSize: 100ul
Description
Low affinity IgG Fc receptor gamma II (Fc-gamma II), also known as CD32, is a member of the immunoreceptor tyrosine-based activating/inhibitory motif (ITAM/ITIM) family. Fc gamma receptors play a critical role in immunity by linking the IgG antibody mediated response with the regulatory functions of the immune system (1). Three classes of Fc gamma receptor exist; Fc gamma RI (CD64, a high affinity receptor), Fc gamma RII, and RIII (CD32 and CD16 respectively, both low affinity receptors). Fc gamma RIIa is an activating receptor, while RIIb1, RIIb2, and RIIb3 are inhibitory receptors generated by alternative splicing (1-2). Fc gamma RIIa is critical for antigen-presenting-cell activation and Fc gamma RIIb downregulates B-cell functions (3). Fc gamma RIIb has also been known to block theinflammatory immune response in mice (4). Once phosphorylated on tyrosine residues, Fc gamma RIIb associates with SHIP to downregulate phagocytosis. | |
Recommended Applications
WB, ICC, FC | | | | Applications and Recommended Dilution Factors | | | | WB | IHC | ICC | FC | IP |
| | 1: 5,000-50,000 | | 1:250-500 | 1:40 | | |
| Species Reactivity* | | | | Human | Mouse | Rat |
| | Positive | Negative | Negative | *Cross reactivity determined by western blot only. | |
| Product Data |
 | | | A. Western blot analysis on Daudi cell lysate using anti-Fc gamma RIIb (C-term) Rabbit Polyclonal (cat. # 1723-1), dilution 1:50,000. | | |
Specificity
A synthetic peptide corresponding to residues in the C-terminus of human Fc gamma RIIb was used as an immunogen. |
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Alternative Names
FCGR2B, CD32, FCG2, IGFR2, Low affinity immunoglobulin gamma Fc region receptor II-b, CDw32 |
Description References
1. Ravetch J, Bolland S. Annu Rev Immunol. 19: 275-90, 2001.
2. Tsubata, T. (1999). Curr Opin Immunol 11, 249
3. Malbec, O., Fridman, W. H. & Daeron, M. (1999).Curr Top Microbiol Immunol 244, 13
4. Marie et al. Immunity 14, 69 |
