Description
A Proline rich protein tyrosine kinase (Pyk2) is a focal adhesion kinase (FAK) related protein kinase protein also known as cell adhesion kinase (CAK), related adhesion focal tyrosine kinase (RAFTK), or calcium-dependent PTK (CADTK) (3.4). Highly expressed in the nervous system, protein tyrosine kinase (Pyk2) is phosphorylated on the tyrosine residue by a variety of G-protein-coupled receptors & extracellular signals that increase the level of Ca + intracellulary (1,2) | |
Recommended Applications
WB, IHC, ICC, IP | | | | Applications and Recommended Dilution Factors | | | | WB | IHC | ICC | FC | IP |
| | 1:500 | 1:250-500 | 1:100 | | 1:80 | |
| Species Reactivity* | | | | Human | Mouse | Rat |
| | Positive | Positive | Positive | *Cross reactivity determined by western blot only. | |
| Product Data |
 |  | | A. Western blot analysis on Jurkat cell lysate using anti-Pyk2 (N-term) RabMAb (catalog #1509-1), dilution 1:5,000 | B. Immunohistochemical analysis of paraffin-embedded human normal brain tissue using anti-Pyk2 (N-term) RabMAb (catalog #1509-1). |  | | | C. Immunofluorescent staining of PC12 cells using anti-Pyk2 (N-term) RabMAb (catalog #1509-1). | | |
Specificity
A synthetic peptide corresponding to residues in the N-terminus of human PYK2 was used as immunogen. The antibody does not cross-react with other FAK family members. |
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Alternative Names
PTK2B, FAK2, PYK2, RAFTK, Protein tyrosine kinase 2 beta, Related adhesion focal tyrosine kinase |
Description References
1. Andreev J., Simon J.-P., Sabatini D.D., Kam J., Plowman G., Randazzo P.A., Schlessinger J.; Identification of a new Pyk2 target protein with Arf-GAP activity.; Mol. Cell. Biol. 19:2338-2350(1999).
2. Schlessinger J., 1995. Protein tyrosine kinase PYK2 involved in calcium-induced regulation of ion channel and MAP kinase functions. Nature376:737-745
3. Candice K. Klingbeil, Christof R. Hauck, et al. Targeting Pyk2 to
4. Avraham H., Park S.Y., Schinkmann K., Avraham S., 2000. RAFTK/Pyk2-mediated cellular signalling. Cell. Signal12:123-133 |
Product References
1. Pape EL, Passeron T, Giubellino A, et al, Microarray analysis sheds light on the dedifferentiating role of agouti signal protein in murine melanocytes via the Mc1r ,PNAS 106 (2009) 1802 - 1807
[Application: WB ] |
