详细说明
FAK Antibody Rabbit Monoclonal Antibody Cat.# 2146-1 Clone ID: EP1831YSwiss Prot: Q05397 Mol Weight: 125 kDaSize: 100ul
Description
Focal adhesion kinase (FAK) is a non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis (1). FAK is composed of a central catalytic domain flanked by large N- and C-terminal regions. FAK is activated by phosphorylation at tyrosine 397 in response to integrin clustering which can be induced by cell adhesion or antibody cross-linking or via G-protein-coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid (2-3). Phosphorylation of FAK Tyr-397 creates a binding site for Src-family kinases, and phosphorylation of FAK Tyr-576/Tyr-577 in the kinase domain activation loop enhances catalytic activity (4). Increased FAK expression has been correlated with the enhanced motility and invasiveness of human tumor cells, as well as with promoting increased cell proliferation (5). | |
Recommended Applications
WB, IHC, ICC, FC | | | | Applications and Recommended Dilution Factors | | | | WB | IHC | ICC | FC | IP |
| | 1:500 - 2000 | 1:100 - 250 | 1:100 - 250 | 1:30 | | |
| Species Reactivity* | | | | Human | Mouse | Rat |
| | Positive | Positive | Positive | *Cross reactivity determined by western blot only. | |
| Product Data |
 |  | | A. Western blot analysis on HeLa cell lysate using anti-FAK1 RabMAb (cat. # 2146-1), dilution 1:500. | B. Immunohistochemical staining of paraffin-embedded human tonsils using anti-FAK RabMAb (cat. #2146-1). | |
Specificity
A synthetic peptide corresponding to residues in human FAK were used as an immunogen |
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Alternative Names
PTK2, FAK, FAK1, Focal adhesion kinase 1, pp125FAK;Protein-tyrosine kinase 2 |
Description References
1. Schaller, M.D. (2001) Biochim. Biophys. Acta 1540, 1
2. Schaller, M.D., et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 5192
3. Salazar, E.P, et al. (1999) J. Biol. Chem. 274, 28371
4. Tremblay L., et al. Int. J. Cancer, 68: 164-171, 1996 |
