详细说明
FADD Phospho (pS194) Antibody Rabbit Monoclonal Antibody Cat.# 2354-1 Clone ID: EPR1816YSwiss Prot: Q61160 Mol Weight: 28 kDaSize: 100ul
Description
Fas-associating protein with death domain (FADD) is an adaptor protein involved in the apoptosis induced by Fas and TNF-R1, and mitogen induced cell proliferation (1). FADD consists of death domain (DD) at the C-terminal and death effector domain (DED) at the N-terminal (2). DD interacts with Fas and TNF-R1, which exposes the DED portion on the FADD. Unmasked DED binds to the DED of pro-caspase-8, which activates the cysteine protease cascade (3). The development of autoimmune lymphoproliferative (lpr) diseases has been linked to inactivation of Fas-FADD interaction (4). Also, the deficiency in FADD can inhibit TRAIL-induced cell apoptosis.
In cancer cells, higher phopsholyation of FADD at serine 194 is expressed compared to normal cells. Phospholyation at serine 194 has been linked to induction of apoptosis by anti-cancer drugs in human protstate cancer cells and it is considered as a valuable maker for human prostate cancer progression (5). | |
Recommended Applications
WB, IP | | | | Applications and Recommended Dilution Factors | | | | WB | IHC | ICC | FC | IP |
| | 1:500 | | | | 1:30 | |
| Species Reactivity* | | | | Human | Mouse | Rat |
| | Positive | Negative | Negative | *Cross reactivity determined by western blot only. | |
| Product Data |
 | | | A. Western blot analysis on Jurkat cell lysates using anti-phosho FADD (pS194) RabMAb (cat. #2354-1), 1:500 dilution. Cells were either (A) untreated (B) treated with calyculin A | | |
Specificity
A synthetic peptide corresponding to residues surrounding serine 194 of human FADD was used as immunogen. |
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Alternative Names
Fadd, Mort1, Protein FADD, FAS-associated death domain protein;FAS-associating death domain-containing protein;Mediator of receptor induced toxicity |
Description References
1. Imtiyaz HZ et al, I J. Biol. Chem., 280(36);31360-67, 2005
2. Chinnaiyan AM et al. Cell 81, 505
3. Boldin MP et al, Cell 85, 803
4. Cohen PL et al. Annu. Rev. Immunol. 9, 243
5. Shimada K, et al. J Pathol 206:423-32, 2005. |
