详细说明
Ezrin/Radixin/Moesin Antibody Rabbit Monoclonal Antibody Cat.# 2180-1 Clone ID: EP2122YSwiss Prot: P15311 Mol Weight: 81/75 kDaSize: 100ul
Description
Erzin, a member of the Ezrin, Radixin, Moesin (ERM) family, is a linker protein located between cell surface receptors, adhesion molecules, and actin cytoskeleton (1-2). Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. Recent cloning of the murine and porcine radixin cDNAs revealed a protein highly homologous to ezrin and moesin (3). oesin (membrane-organizing extension spike protein) has previously been characterized as a possible receptor protein for heparan sulfate and also as a cytoskeletal linker protein that stabilizes cell surface microvilli, filopodia and lamellipodia. Data indicate that moesin is identical to the 77-kDa band that copurifies with ezrin in its isolation from human placenta (4). | |
Recommended Applications
WB, IHC, ICC, IP | | | | Applications and Recommended Dilution Factors | | | | WB | IHC | ICC | FC | IP |
| | 1:1000 | 1:100 - 250 | 1:250 - 500 | | 1:40 | |
| Species Reactivity* | | | | Human | Mouse | Rat |
| | Positive | Positive | Positive | *Cross reactivity determined by western blot only. | |
| Product Data |
 |  | | A. Western blot analysis on (A) A431 and (B) MCF7 cell lysates using anti- Ezrin/Radixin/Moesin RabMAb (cat. # 2180-1), dilution 1:1000. | B. Immunohistochemical analysis of paraffin-embedded human breast adenocarcinoma using anti-EzrinRadixinMoesin RabMAb. |  | | | C. Immunofluorescent staining of HeLa cells using anti-EzrinRadixinMoesin (cat. #2180-1). | | |
Specificity
A synthetic peptide corresponding to residues surrounding Thr566 (Ezrin 81 kDa) Thr564 (Radixin 81 kDa) and Thr558 (Moesin 75 kDa) of human Ezrin, Radixin and Moesin was used as an immunogen. This antibody will detect all 3 proteins. |
Storage Buffer & Conditions
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Alternative Names
EZR, VIL2, Ezrin, p81;Cytovillin;Villin-2 |
Description References
1. Bretscher, A., Edwards, K., and Fehon, R. G. (2002) Nat. Rev. Mol. Cell. Biol. 3, 586-599
2. Tsukita, S., and Yonemura, S. (1999) J. Biol. Chem. 274, 34507-34510
3. Wilgenbus KK, et al. Genomics 16(1):199-206, 1993
4. Lankes WT, et al. Proc Natl Acad 88(19):8297-301, 1991 |
