Description:
GCpro Taq DNA Polymerase is a versatile and easy-to use enzyme, with powerful advantages for any PCR application. GCpro Taq DNA Polymerase is prepared from a recombinant clone expressed in E. coli, containing the DNA polymerase I gene from Thermococcus litoralis. The enzyme includes a highly processive 5-3 DNA polymerase activity. Its inherent 3->5 proofreading activity results in greatly increased fidelity compared to Taq DNA Polymerase. The enzyme includes an optimized reaction buffer, which enables the amplification of GC-rich templates and templates with secondary structures that interfere with the PCR process. GCpro Taq DNA Polymerase promotes very robust synthesis of longer GC-rich amplification products. GCpro Taq DNA Polymerase has all the properties of regular Taq DNA Polymerases for every standard PCR application. It is versatile, user-friendly and compatible with all PCR applications even the amplification of GC-rich and other complex templates, site-directed mutagenesis, and TA cloning.

Applications:
GCpro Some of the applications of GenScript GCpro Taq DNA Polymerase are as follows:

• Excellent yields with GC-rich and other complex templates
• Site-directed mutagenesis
• Higher fidelity than standard Taq DNA Polymerase
• TA cloning
• High specific activity
• High specificity due to combination of enzyme and unique PCR buffer

Contents:

• GCpro Taq DNA polymerase
• dNTP 10 mM 50ul
• Lyophilized positive control 50 rxns (add 50 μl ddH₂O before use, 1 μl/rxn)
• 1.5 ml, 2X buffer, 500 mM Tris-HCl (pH8.9 at 25 ℃), 0.5% Tween 20, 0.5% Nonidet P40, 1 mg/ml BSA ,with 3 mM MgCl₂

Activity:
GenScript GCpro Taq DNA Polymerase shows very good fidelity and an average error rate around 2X10^-6. GCpro Taq DNA Polymerase has no 5->3 exonuclease activity, but it does have the extendase activity necessary for TA cloning.

Unit Definition:
One unit is defined as the amount of enzyme that can incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74 ℃.

Storage Buffer and Concentration:
Supplied in 5 units/μl in 50 mM Tris-HCl (pH8.1), 0.1 mM EDTA, 1 mM DTT, 0.1% (v/v) nonidet P40, 0.1% (v/v) tween 20 and 50% (v/v) glycerol.

Storage:
Store the enzyme at -20 ℃. It will remain stable for at least one year if stored properly.