骨切片使用于采用传统染色法或Crosslaps? for culture ELISA法对」破骨细胞骨吸收进行精确的体外评估
Bone slices* are accurate in vitro assessment of osteoclastic bone resorption either by traditional staining or by use of the CrossLaps® for Culture ELISA assay.
Bone is a dynamic tissue that is remodelled through life. It is believed that special factors are present in bone, which may affect the activity of osteoclast. Thus at IDS we use high quality bone, not dentine, for all our research.
Each batch of bone slices is tested for quality by resorption assays for verification of osteoclastic ability to make pits on these exact bone slices. Subsequently, the supernatant is measured by the Crosslaps® for Culture ELISA kit and only if both tests are positive the bone slices are accepted for further resorption assays.
Assay Details:
Storage
The bone slices are stored in 70% ethanol at 4°C. However, for shorter time periods and during transport room temperature is adequate for retaining the quality of the bone slices.
Handling
We recommend that our bone slices are washed 3 times in 10% serum containing medium before they are transferred to the 96 well plates.
Culture
Usually resorption experiments lasts 72 hours, however already after 16 or 24 hours after addition compound to osteoclast cultures a significant differences are observed. Even after 8 hours a significant difference can be observed in optimised assays.
