Bis-Tris propane is a zwitterionic buffer that is used in biochemistry and molecular biology. It has an unusually wide buffering range, from approximately pH 6 to 9.5, because its two pKa values are close in value.
A review of DNA polymerase fidelity and its role in the polymerase chain reaction (PCR) discusses the use of Bis-Tris propane as a suitable buffer. The use of a Bis-Tris propane/HCl buffer for the stabilization of farnesyl diphosphate isolated from a mutant strain of Saccharomyces cerevisiae has been described.
BisTris propane buffer has been used in a study of the effects of buffer identity on electric signals of lightexcited bacteriorhodopsin. An investigation of the MgATPase activity of the myosin subfragment monomer in Bis-Tris propane buffer has been reported. The effect of buffer identity on the kinetics of the restriction enzyme EcoR V has been studied in various buffers, including Bis-Tris propane.
Bis-Tris propanehas been used in protein crystallization, such as in the crystallization of glutamate dehydrogenase from Thermotoga maritima and of pullulanase type I from Fervidobacteriumpennivorans Ven The analysis of Langmuir and Langmuir-Blodgett monolayers of organophosphorus acid anhydrolase in different subphases, including Bis-Tris propane buffer, has been reported.The preparation and phosphodiester hydrolysis activity of complexes of lanthanum and Bis-Tris propane have been investigated.
