Isolation date: 1964
(The HT-29 line was isolated from a primary tumor in 1964 by J. Fogh using the explant culture method.)

urokinase receptor (u-PAR); vitamin D (moderate expression)
human adrenergic alpha2A [23560]
human adrenergic alpha2A [23560]
urokinase receptor (u-PAR)
vitamin D (moderate expression)

Yes, in nude mice; forms well differentiated adenocarcinoma consistent with colonic primary (grade I); tumors also form in steroid treated hamsters

Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5

modal number = 71; range = 68 to 72.
The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.

44 years adult

Caucasian

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature

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