efficacy testing [92587]
testing [92346] [92389]
transfection host (Roche FuGENE® Transfection Reagents)
viruscide testing [92579] [92582]

negative

Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal.

14 weeks gestation

Caucasian

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0C

Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temerpature

22893: Jacobs JP , et al. Characteristics of a human diploid cell designated MRC-5. Nature 227: 168-170, 1970. PubMed:
26036: Proc. Symp. Human Diploid Cells. 227: Zagreb, Yugoslavia: Yugoslav Acad. Sci. Arts; 1970, pp. 43-55.
26037: Holliday R , Tarrant GM . Altered enzymes in ageing human fibroblasts. Nature 238: 26-30, 1972. PubMed:
26038: Lewis CM , Tarrant GM . Error theory and ageing in human diploid fibroblasts. Nature 239: 316-318, 1972. PubMed:
32288: Landers JE , et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed:
32696: Kaur A , et al. Cytotoxic T-lymphocyte responses to cytomegalovirus in normal and simian immunodeficiency virus-infected Rhesus macaques. J. Virol. 70: 7725-7733, 1996. PubMed:
92346: Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia: Standards Australia; Standards Australia AS ISO 10993.5-2002.
92389: Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland): International Organization for Standardization/ANSI; ISO ISO 10993-5:1999.
92579: Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA: ASTM International; ASTM Standard Test Method E 1838-02.
92582: Standard Test Method for Evaluation of Handwashing Formulation for Virus-Eliminating Activity using the Entire Hand. West Conshohocken, PA: ASTM International; ASTM Standard Test Method E 2011-99.
92587: Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA: ASTM International; ASTM Standard Test Method E 2197-02.