COLO 205(结肠癌▽细胞)_教育装备▲采购网

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品　　牌：ATCC

产品型号：CCL-222 (株)

制造厂商：上海锐聪科技发展＠有限公司

所在地区：上海

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COLO 205(结肠癌细胞)

COLO 205(结肠癌细胞)

Cell Biology
ATCC^® Number:	CCL-222™	Price:	$203.00
Designations:	COLO 205	Depositors:	GE Moore
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	mixed, adherent and suspension
Organism:	Homo sapiens (human)	Morphology:	epithelial

Source:	Organ: colon Disease: colorectal adenocarcinoma Tumor stage: Dukes type D Derived from metastatic site: ascites
Cellular Products:	carcinoembryonic antigen (CEA) 1.5 to 4.1 ng/10 exp6 cells/10 days; keratin; interleukin 10 (IL-10, interleukin-10)
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Applications:	transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic:	Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Reverse Transcript:	negative
DNA Profile (STR):	Amelogenin: X CSF1PO: 11,12 D13S317: 10,12 D16S539: 12,13 D5S818: 10,13 D7S820: 9,10 THO1: 8,9 TPOX: 11 vWA: 15
Cytogenetic Analysis:	The stemline chromosome number is hypertriploid and 10-11 marker chromosomes (M1, M2, M4, M5, M6 and 5 others) were common in monosomic, disomic and trisomic condition although most of these markers were monosomic.
Isoenzymes:	ES-D, 1-2; G6PD, B; PEP-D, 1; PGD, A; PGM1, 1-2; PGM3, 1-2
Age:	70 years
Gender:	male
Ethnicity:	Caucasian
Comments:	This line was isolated in 1975 by T.U. Semple, et al. from ascitic fluid of a 70-year-old Caucasian male with carcinoma of the colon. The patient had been treated with 5-fluorouracil for 4-6 weeks before removal of the fluid specimen. [1046] The cells are CSAp negative (CSAp-). The line was derived from tissue from the same patient as COLO 201 (ATCC CCL-224). The cells are positive for keratin by immunoperoxidase staining. COLO 205 cells express a 36000 dalton cell surface glycoprotein related to the GA733-2 tumor associated antigen.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C
Subculturing:	Protocol: Shake flask, transfer floating cells inot a new flask. Cells remaining attached may be removed using a standard trypsinization protocol. Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium renewal: Every 2 to 3 days
Preservation:	Freeze medium: Complete culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2001 recommended serum: ATCC 30-2020 derived from same individual: ATCC CCL-224
References:	1046: Semple TU , et al. Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 38: 1345-1355, 1978. PubMed: 565251 22511: Gastl GA , et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 22861: Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 41500: Bjork P , et al. Isolation, partial characterization, and molecular cloning of a human colon adenocarcinoma cell-surface glycoprotein recognized by the C215 mouse monoclonal antibody. J. Biol. Chem. 268: 24232-24241, 1993. PubMed:

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COLO 205(结肠癌细→胞)