immunoglobulin; monoclonal antibody; against HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl Coenzyme A reductase, HMG CoA reductase)
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Animals were immunized with cell membranes from UT-1 (a cell line derived from the CHO K1 cell line). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with HMG-CoA reductase (the rate limiting enzyme in the cholesterol biosynthetic pathway). Tested and found negative for ectromelia virus (mousepox).
ATCC complete growth medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; fetal bovine serum, 10%
Subculturing:
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium renewal: Every 2 to 3 days
References:
1621: Liscum L , et al. Regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase and its mRNA in rat liver as studied with a monoclonal antibody and a cDNA probe. J. Biol. Chem. 258: 8450-8455, 1983. PubMed:
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