transfection host (technology from amaxa
Roche FuGENE® Transfection Reagents)

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Growth Conditions: The myoblastic component of this line will be depleted rapidly if the cells are allowed to become confluent.

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase

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