Neuro-2A(脑神经瘤细∑胞)_教育装↓备采购网

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品　　牌：ATCC

产品型号：CCL-131 (株)

制造厂商：上海锐聪科技发展有限公司

所在地区：上海

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Neuro-2A(脑神经瘤细胞)

Neuro-2A(脑神经瘤细胞)

Cell Biology
ATCC^® Number:	CCL-131™	Price:	$203.00
Designations:	Neuro-2a	Depositors:	RJ Klebe
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Mus musculus (mouse)	Morphology:	neuronal and amoeboid stem cells

Source:	Organ: brain Cell type: neuroblast Disease: neuroblastoma
Cellular Products:	acetylcholinesterase tubulin
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Applications:	transfection host (technology from amaxa Roche FuGENE® Transfection Reagents)
Virus Susceptibility:	Herpes simplex virus Vesicular stomatitis virus
Virus Resistance:	Poliovirus 1
Reverse Transcript:	negative
Antigen Expression:	H-2, a haplotype; Mus musculus, expressed
Cytogenetic Analysis:	modal number = 95; range = 59 to 193. Karyotype unstable within a stemline range of 94 to 98 chromosomes. All the cells contain 6 to 10 large chromosomes with median or submedian centromeres and 2 to 4 minute chromosomes.
Genotype:	albino
Strain:	A
Comments:	Clone Neuro-2a was established by R.J. Klebe and F.H. Ruddle from a spontaneous tumor of a strain A albino mouse. This tumor line, designated C1300, was obtained from the Jackson Laboratory, Bar Harbor, Maine [22161]. Neuro-2a cells produce large quantities of microtubular protein which is believed to play a role in a contractile system which is responsible for axoplasmic flow in nerve cells. The cell line has been used for studies on the mechanism of vinblastine precipitation of microtubular protein, the kinetics of GTP binding to isolated protein, the turnover of microtubules in vivo, and the synthesis and assembly of microtubular protein [PubMed: ]. Tested and found negative for ectromelia virus (mousepox).
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium renewal: 1 to 2 times per week
Preservation:	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003 recommended serum: ATCC 30-2020 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++): ATCC 30-2101 Cell culture tested DMSO: ATCC 4-X
References:	1023: Olmsted JB , et al. Isolation of microtubule protein from cultured mouse neuroblastoma cells. Proc. Natl. Acad. Sci. USA 65: 129-136, 1970. PubMed: 22161: Klebe RJ , Ruddle FH . Neuroblastoma: Cell culture analysis of a differentiating stem cell system. J. Cell Biol. 43: 69A, 1969. 29352: Naslavsky N , et al. Characterization of detergent-insoluble complexes containing the cellular prion protein and its scrapie isoform. J. Biol. Chem. 272: 6324-6331, 1997. PubMed: 29861: Kaneko K , et al. Evidence for protein X binding to a discontinuous epitope on the cellular prion protein during scrapie prion propagation. Proc. Natl. Acad. Sci. USA 94: 10069-10074, 1997. PubMed: 32459: Maestrini E , et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed:

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Neuro-2A(脑神经瘤细胞)