transfection host (technology from amaxa
Roche FuGENE® Transfection Reagents)

Yes, in nude mice [1050]
(subcutaneous myeloid tumors)
Yes, in semi-solid media [1050]

myc +

The stemline chromosome number is pseudodiploid with the 2S component occurring at 6.2%. Five markers (M2 through M6) were common to most S metaphases. DMs, which varied in numbers per cell, occurred in all metaphases karyotyped. HSR chromosomes were not detected.

36 years

Caucasian

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase

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