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                PLC/PRF/5(肝癌亚力山大细胞)

                PLC/PRF/5(肝癌亚力山大细胞)
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                • PLC/PRF/5(肝癌亚力山大细胞)
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                ATCC
                CRL-8024 (株)
                上海锐聪科技发展有限公司
                上海
                详细说明

                PLC/PRF/5(肝癌亚力山大细胞)

                PLC/PRF/5(肝癌亚力山大细胞)
                 
                Cell Biology
                ATCC® Number: CRL-8024™ Price: $255.00
                Designations:
                PLC/PRF/5
                Depositors:
                WJ McAleer
                2 [Cells Contain HEPATITIS B]
                Shipped:
                frozen
                Medium & Serum:
                Growth Properties:
                adherent
                Organism:
                Homo sapiens (human)
                Morphology:
                epithelial
                 
                Source:
                Organ: liver
                Cell type: Alexander cells
                Disease: hepatoma
                Cellular Products:
                hepatitis virus B surface antigen (HBsAg)
                Permits/Forms:
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                This material is cited in a U.S. and/or other Patent or Patent Application, and may not be used to infringe on the patent claims.
                DNA Profile
                (STR):
                Amelogenin: X
                CSF1PO: 10
                D13S317: 11,12
                D16S539: 13
                D5S818: 12
                D7S820: 9,11
                THO1: 8
                TPOX: 8
                vWA: 15,16
                Comments:
                The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline.
                The cells secrete HBsAg.
                Propagation:
                ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                Temperature: 37.0C
                Subculturing:
                Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
                Add fresh culture medium, aspirate and dispense into new culture flasks.
                Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

                Medium renewal: Twice per week
                Preservation:
                culture medium 95%; DMSO, 5%
                Related Products:
                Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003
                recommended serum: ATCC 30-2020
                References:
                22189: Monjardino J , Crawford E . Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed: 223321
                22198: MacNab GM , et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer 34: 509-515, 1976. PubMed: 187208
                22236: Skelly J , et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138
                22244: Science 209: 407, 1980.
                22265: S. Afr. J. Med. Sci. 41: 8-9, 1976.
                23123: Gilligan A , et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed:
                23525: Alexander JJ , et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J. 50: 2124-2128, 1976. PubMed: 63998

                 

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