SW579(人甲〖状腺癌细胞)_教育装备采购〒网

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品　　牌：ATCC

产品型号：HTB-107 (株)

制造厂商：上海锐聪科技发展△有限公司

所在地区：上海

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SW579(人甲状腺癌细胞)

SW579(人甲状腺癌细胞)

Cell Biology
ATCC^® Number:	HTB-107™	Price:	$330.00
Designations:	SW579 [SW 579; SW-579]	Depositors:	A Leibovitz
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	epithelial

Source:	Organ: thyroid Disease: squamous cell carcinoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Isolation:	Isolation date: 1973
Tumorigenic:	Yes, forms tumors in nude mice (The cells produce grade III malignant spindle and giant cell tumors.)
Antigen Expression:	blood type O; Rh+
DNA Profile (STR):	Amelogenin: X CSF1PO: 13 D13S317: 13 D16S539: 11 D5S818: 11 D7S820: 8,9 THO1: 8,9.3 TPOX: 8,10 vWA: 14,18
Isoenzymes:	AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 1-2; PGM1, 1-2; PGM3, 1
Age:	59 years
Gender:	male
Ethnicity:	Caucasian
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C Atmosphere: air, 100%
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium renewal: 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2008 recommended serum: ATCC 30-2020
References:	22536: Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

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SW579(人甲状腺癌细胞)