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RKO(结肠腺╲癌细胞)

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产品报价：面议

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产品热度：加载中

品　　牌：ATCC

产品型号：CRL-2577 (株)

制造厂商：上海锐聪科技发展【有限公司

所在地区：上海

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RKO(结肠腺癌细胞)

RKO(结肠腺癌细胞)

Cell Biology
ATCC^® Number:	CRL-2577™	Price:	$255.00
Designations:	RKO	Depositors:	MC Hollander AJ Fornace
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	epithelial

Source:	Organ: colon Disease: carcinoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Receptors:	urokinase receptor (u-PAR) [51462]
Tumorigenic:	Yes, in nude mice Yes, in soft agar
Oncogene:	p53 + (wild type) [51463]
Comments:	RKO is a poorly differentiated colon carcinoma cell line developed by Michael Brattain. RKO cells contain wild-type p53 but lack endogenous human thyroid receptor nuclear receptor (h-TRbeta1). The level of p53 protein is higher in RKO (ATCC CRL-2577) cells than in RKO-E6 (ATCC CRL-2578) cells. [53331] The RKO cell line is the parental cell line (isogenic) of RKO-E6 (ATCC CRL-2578) and RKO-A545-1 (ATCC CRL-2579). It can be used as the control cell line for investigating the effects of p53 and gadd45 on cellular parameters.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C
Subculturing:	Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:12 is recommended Medium renewal: Every 2 to 3 days
Preservation:	culture medium 95%; DMSO, 5%
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003 recommended serum: ATCC 30-2020 derivative: ATCC CRL-2578 derivative: ATCC CRL-2579
References:	51462: Boyd D , et al. Determination of the levels of urokinase and its receptor in human colon carcinoma cell lines. Cancer Res. 48: 3112-3116, 1988. PubMed: 51463: Smith ML , et al. Involvement of the p53 tumor suppressor in repair of u.v.-type DNA damage. Oncogene 10: 1053-1059, 1995. PubMed: 53329: Brattain MG , et al. Heterogeneity of human colon carcinoma. Cancer Metastasis Rev. 3: 177-191, 1984. PubMed: 53331: Bhat MK , et al. Tumor suppressor p53 is a negative regulator in thyroid hormone receptor signaling pathways. J. Biol. Chem. 272: 28989-28993, 1997. PubMed:

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