FaDu(人咽『鳞癌细胞㊣№╱)_教育装备采∴购网

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品　　牌：ATCC

产品型号：HTB-43 (株)

制造厂商：上海锐聪科技发展有限公司

所在地区：上海

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FaDu(人咽鳞癌细胞)

FaDu(人咽鳞癌细胞)

Cell Biology
ATCC^® Number:	HTB-43™	Price:	$255.00
Designations:	FaDu	Depositors:	SR Rangan
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	epithelial

Source:	Organ: pharynx Disease: squamous cell carcinoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Applications:	transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic:	Yes, in nude mice; forms well differentiated epidermoid carcinoma (grade I)
Cytogenetic Analysis:	(P16) hypodiploid to hypertriploid with modal number = 64
Isoenzymes:	AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 2; PGM1, 2; PGM3, 1
Age:	56 years
Gender:	male
Ethnicity:	Caucasian
Comments:	The FaDu line was established in 1968 from a punch biopsy of an hypopharyngeal tumor removed from a Hindu patient. The established line was found to contain bundles of tonofilaments in the cell cytoplasm and desmosomal regions were prominent at cell boundaries.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C
Subculturing:	Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium renewal: 2 to 3 times per week
Preservation:	Culture medium, 95%; DMSO, 5%
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003 recommended serum: ATCC 30-2020
References:	22536: Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 22792: Rangan SR . A new human cell line (FaDu) from a hypopharyngeal carcinoma. Cancer 29: 117-121, 1972. PubMed: 23093: Faust JB , Meeker TC . Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 23218: Giard DJ , et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed:

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FaDu(人咽鳞癌细胞)