MG-63(骨肉瘤细胞ㄨ)_教育装备▆采购网↘

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品　　牌：ATCC

产品型号：CRL-1427 (株)

制造厂商：上海锐聪↓科技发展有限公司

所在地区：上海

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MG-63(骨肉瘤细胞)

MG-63(骨肉瘤细胞)

Cell Biology
ATCC^® Number:	CRL-1427™	Price:	$203.00
Designations:	MG-63	Depositors:	A Billiau
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	fibroblast

Source:	Organ: bone Disease: osteosarcoma
Cellular Products:	interferon
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Applications:	transfection host (technology from amaxa Roche FuGENE® Transfection Reagents)
Receptors:	transforming growth factor beta (TGF-beta) RI, expressed transforming growth factor beta (TGF-beta) RII, expressed
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11 D16S539: 11 D5S818: 11,12 D7S820: 10 THO1: 9.3 TPOX: 8,11 vWA: 16,19
Cytogenetic Analysis:	This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells.
Age:	14 years
Gender:	male
Ethnicity:	Caucasian
Comments:	High levels of interferon production can be induced using polyinosinic - polycytidylic acid, cycloheximide and actinomycin D.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%. Temperature: 37.0C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium renewal: 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003
References:	1058: Billiau A , et al. Human interferon: mass production in a newly established cell line, MG-63. Antimicrob. Agents Chemother. 12: 11-15, 1977. PubMed: 883813 23014: Takeuchi Y , et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. PubMed: 32445: Yee A , et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996. PubMed: 85506

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MG-63(骨肉瘤细胞)