HEC-1-B(子宫内膜腺＠癌细胞)_教▓育装备采购网

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品　　牌：ATCC

产品型号：HTB-113 (株)

制造厂商：上海锐聪▽科技发展有限公司

所在地区：上海

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HEC-1-B(子宫内膜腺癌细胞)

HEC-1-B(子宫内膜腺癌细胞)

Cell Biology
ATCC^® Number:	HTB-113™	Price:	$255.00
Designations:	HEC-1-B	Depositors:	H Kuramoto
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	epithelial

Source:	Organ: uterus Tissue: endometrium Disease: adenocarcinoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Applications:	transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic:	Yes, in nude mice (The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma (grade II).) Yes, in steroid-treated hamsters
Antigen Expression:	Blood Type B; Rh+
DNA Profile (STR):	Amelogenin: X CSF1PO: 10,12 D13S317: 11,16 D16S539: 11,12 D5S818: 11,13 D7S820: 9,11 THO1: 6,7 TPOX: 8,11 vWA: 18
Cytogenetic Analysis:	diploid to tetraploid with large submetacentric marker
Isoenzymes:	AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 2; PGM1, 1; PGM3, 1-2
Age:	71 years
Gender:	female
Comments:	This is a substrain of HEC-1-A (see ATCC HTB-112) isolated in 1968 by H. Kuramoto. Unlike HEC-1-A, this substrain exhibited a stationary growth period between the 135th and 190th days in culture and appeared on recovery to be flattened and more pavement patterned than the parent line. Futhermore, the predominant complement of chromosomes was double that observed for the parent line.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C
Subculturing:	Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium renewal: Every 2 to 3 days
Preservation:	Culture medium, 95%; DMSO, 5%
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003 recommended serum: ATCC 30-2020
References:	22449: Kuramoto H . Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line. Acta Obstet. Gynaecol. Jpn. 19: 47-58, 1972. PubMed: 22536: Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 23069: Presta M , et al. Modulation of plasminogen activator activity in human endometrial adenocarcinoma cells by basic fibroblast growth factor and transforming growth factor beta. Cancer Res. 48: 6384-6389, 1988. PubMed: 23541: Kuramoto H , et al. Establishment of a cell line of human endometrial adenocarcinoma in vitro. Am. J. Obstet. Gynecol. 114: 1012-1019, 1972. PubMed: 32299: St. Geme JW , et al. Characterization of the genetic locus encoding Haemophilus influenzae type b surface fibrils. J. Bacteriol. 178: 6281-6287, 1996. PubMed: 33112: Schramm N , et al. Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells. Infect. Immun. 64: 1208-1214, 1996. PubMed:

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HEC-1-B(子宫内膜腺癌细胞)