The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

heat stable enterotoxin (Sta, E. coli), expressed
epidermal growth factor (EGF), expressed

Yes, in nude mice; forms moderately well differentiated adenocarcinoma consistent with colonic primary (grade II)

AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1; Me-2, 1; PGM1, 1; PGM3, 1

male

Upon reaching confluence, the cells express characteristics of enterocytic differentiation [PubMed ID: ]. Caco-2 cells express retinoic acid binding protein I and retinol binding protein II [PubMed ID: ].

Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.The recommended inoculum is 1 X 10(4) viable cells/cm2. Subculture cells when they are about 80% confluent, at a cell concentration between 8 X 10(4) and 1 X 10(5) cell/cm2.
6. Incubate cultures at 37C.

Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium renewal: 1 to 2 times per week

about 62 hours

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