| Designations: | JAR | Depositors: | RA Pattillo |
| | 1 | Shipped: | frozen |
| Medium & Serum: | | Growth Properties: | adherent |
| Organism: | Homo sapiens (human) | Morphology: | epithelial |
| |
| Source: | Organ: placenta
Disease: choriocarcinoma |
| Cellular Products: | estrogen; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); hCG production averages 22.5 ng/ml after reculturing |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| Related Cell Culture Products |
| Isolation: | (The JAR line was established by R. A. Pattillo and associates directly from a trophoblastic tumor of the placenta.) |
DNA Profile
(STR): | Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 11
D16S539: 9,10
D5S818: 10,11
D7S820: 10,11
THO1: 6,7
TPOX: 8,11
vWA: 16,18 |
Cytogenetic
Analysis: | This is probably a pseudotriploid human cell line with the modal chromosome number of 68, occurring in 24% of cells, but cells with both 69 (22%) and 70 (18%) chromosome counts also occurred frequently. Cells with higher ploidies occurred at 7.0%.
Karyotypes were extremely complex. Consistently there were 20 to 25 marker chromosomes (>30% of total chromosome content) per cell. Most marker chromosomes had complex structural rearrangements, and the origin of chromosome segments of these markers often defied identification. Among the frequently found markers were 8p+, 11p, a large metacentric [?t(3qter--3q12::?--C--?::3q12--3qter)] der(?13)T(1;?13) (p13;?q14), and many others. There was only one normal X chromosome. Normal N3 and N13 were not found. |
| Isoenzymes: | AK-1, 1; ES-D, 2; G6PD, B; GLO-I, 1; PGM1, 1-2; PGM3, 1-2 |
| Age: | fetus |
| Gender: | male |
| Ethnicity: | Caucasian |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Subculturing: | Protocol: - Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium renewal: Twice per week |
| Preservation: | Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2001 recommended serum: ATCC 30-2020 |
| References: | 2156: Pattillo RA , et al.. The Jar cell line -- continuous human multihormone production and controls. In Vitro 6: 398-399, 1971. |
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