negative

Occasional polyploids. Several marker chromosomes were observed along with frequent minutes, and often 2 large chromosomes with subterminal centromeres. HeLa Marker Chromosomes: One copy of M2, two-four copies of M3 and one copy of M4 as revealed by G-banding patterns.

Yes

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase

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