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                MOLT-4(急性淋巴母细胞白血病细胞)

                MOLT-4(急性淋巴母细胞白血病细胞)
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                • MOLT-4(急性淋巴母细胞白血病细胞)
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                ATCC
                CRL-1582 (株)
                上海锐聪科技发展有限公司
                上海
                详细说明

                MOLT-4(急性淋巴母细胞白血病细胞)

                MOLT-4(急性淋巴母细胞白血病细胞)
                 
                Cell Biology
                ATCC® Number: CRL-1582™ Price: $203.00
                Designations:
                MOLT-4
                Depositors:
                J Minowada
                1
                Shipped:
                frozen
                Medium & Serum:
                Growth Properties:
                suspension
                Organism:
                Homo sapiens (human)
                Morphology:
                lymphoblast
                 
                Source:
                Cell type: T lymphoblast
                Disease: acute lymphoblastic leukemia
                Cellular Products:
                high levels of terminal deoxynucleotidyl transferase (TdT) are produced [22735]
                Permits/Forms:
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                Applications:
                Tumorigenic:
                Yes, in untreated nude mice, anti lymphocyte serum treated mice and X-irradiated mice [22550]
                Antigen Expression:
                CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%)
                Cytogenetic
                Analysis:
                This is a human cell line with the hypertetraploid chromosome number. The modal chromosome number was 95 occurring in 24% of cells. The rate of cells with higher ploidies was 0.8%. The 6q- and t(7;7) were paired and also common to all cells. The i(17q) was single and occurred only in some cells. Most normal chromosomes had four copies per cell except for the paired N7, X and Y, and two to three copies for N17.
                Age:
                19 years
                Gender:
                male
                Comments:
                The line was established from cells taken from a patient in relapse. [22524]
                The patient had received prior multidrug chemotherapy. [22524]
                There is a G -> A mutation at codon 248 of the p53 gene. [23322]
                P53 is not expressed. [23322]
                The cells do not produce immunoglobulin or Epstein-Barr virus. [22524]
                MOLT-4 was derived from the same patient as the MOLT-3 cell line (ATCC CRL-1552). [22524]
                The line was originally contaminated with mycoplasma but has been cured with antibiotics.
                Propagation:
                ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                Temperature: 37.0C
                Subculturing:
                Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start new cultures at 4 X 10(5) cells/ml and subculture before the cell density reaches 2 X 10(6) cells/ml.

                Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density)
                Preservation:
                Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
                Storage temperature: liquid nitrogen vapor phase
                Related Products:
                Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2001
                recommended serum: ATCC 30-2020
                derived from same individual: ATCC CRL-1552
                References:
                22524: Minowada J , et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed:
                22550: Ohsugi Y , et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed:
                22735: Mertelsmann R , et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed:
                23322: Rodrigues NR , et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed:
                23337: Sandstrom PA , Buttke TM . Autocrine production of extracellular catalase prevents apoptosis of the human CEM T-cell line in serum-free medium. Proc. Natl. Acad. Sci. USA 90: 4708-4712, 1993. PubMed:

                 

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