SCaBER(膀胱鳞癌【细胞)_教育装╱备采购网

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品　　牌：ATCC

产品型号：HTB-3 (株)

制造厂商：上海锐聪科技发展有限公司

所在地区：上海

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SCaBER(膀胱鳞癌细胞)

SCaBER(膀胱鳞癌细胞)

Cell Biology
ATCC^® Number:	HTB-3™	Price:	$330.00
Designations:	SCaBER	Depositors:	C OToole
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	epithelial

Source:	Organ: urinary bladder Disease: squamous cell carcinoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Tumorigenic:	Yes, epidermoid carcinoma forms in nude mice
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 11,13 D13S317: 11,12 D16S539: 11,12 D5S818: 12,13 D7S820: 8,10 THO1: 7,9 TPOX: 9,11 vWA: 16
Cytogenetic Analysis:	2n = 46. The stemline chromosome number is hypotriploid with the 2S component occurring at 9.2%. Eleven markers [t(1;?) del (1)(p13), t(3;?), i(4q), t(8;?), t(9;?), M6, t(1;11), t(21;22), M13, and t(20;?)] and 1 to 3 small chromosome fragments were common to most S metaphases. At least one Y chromosome was always detectable. Number 3 was nullisomic. Neither homogenously staining regions (HSR) nor double minutes (DM) were seen.
Isoenzymes:	AK-1, 1; ES-D, 1; G6PD, A; GLO-I, 1-2; Me-2, 2; PGM1, 1-2; PGM3, 1-2
Age:	58 years
Gender:	male
Ethnicity:	Black
Comments:	Contains the unusual type A isoenzyme of glucose-6-phosphate dehydrogenase.
Propagation:	ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earles BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10% Temperature: 37.0C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing:	Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium renewal: 2 to 3 times per week
Preservation:	Culture medium, 95%; DMSO, 5%
References:	21849: OToole C . Human bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitro. In: Webber, M.M.; Sekely, L.I., eds., editor. In vitro models for cancer research. vol. IV: Boca Raton, FL: CRC Press; pp. 103-125. 22852: OToole C , et al. A cell line (SCABER) derived from squamous cell carcinoma of the human urinary bladder. Int. J. Cancer 17: 707-714, 1976. PubMed: 947851 24381: Fogh J . Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

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SCaBER(膀胱鳞癌细胞)