SK-N-MC(神经上皮瘤细◆胞)

产品报价：面议

留言咨询

产品热度：加载中

品　　牌：ATCC

产品型号：HTB-10 (株)

制造厂商：上海锐聪科技发展有限公︽司

所在地区：上海

详细说明

SK-N-MC(神经上皮瘤细胞)

SK-N-MC(神经上皮瘤细胞)

Cell Biology
ATCC^® Number:	HTB-10™	Price:	$203.00
Designations:	SK-N-MC	Depositors:	G Trempe LJ Old
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	epithelial

Source:	Organ: brain Disease: neuroepithelioma Derived from metastatic site: supra-orbital area
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Restrictions:	The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Applications:	transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic:	Yes, in hamster cheek Yes, in nude mice
Antigen Expression:	Blood Type O; Rh+
DNA Profile (STR):	Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 12 D5S818: 11 D7S820: 8 THO1: 9.3 TPOX: 9,11 vWA: 17,18
Cytogenetic Analysis:	The cell line is a pseudodiploid human female (XX), with chromosome counts in the diploid range and a modal chromosome number of 46. Normal chromosomes N3 and N10 are absent, and many (N1, N2, N4, N15, N16, N17, N21, and N22) are monosomic. Normal chromosome N8 is most often tetrasomic. The remainder of normal chromosomes were usually paired. Numerous marker chromosomes are present including: 1p+, der(3)t(2;3)(q24;q27), del(4)(p12), 11q+, del(2)(q23), ampl.(17)(p12), ampl.(16)(q13), del(15)(q13q22), 21p+, iso(3q), del(22)(q11q13). Marker chromosomes M2 and M3 appear to us to be identical to two markers (M4 and M3, respectively) described by R.C. Seeger, et al.for this cell line.
Isoenzymes:	AK-1, 1; ES-D, 2; G6PD, B; GLO-I, 1-2; Me-2, 2; PGM1, 1; PGM3, 1-2
Age:	14 years
Gender:	female
Ethnicity:	Caucasian
Comments:	This is one of two cell lines (see ATCC HTB-11) of neurogenic origin derived by J.L. Biedler. SK-N-MC was isolated in September of l971 and was found to have moderate dopamine - beta - hydroxylase activity as well as formaldehyde induced fluorescence indicative of intracellular catecholamines.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium renewal: 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003 recommended serum: ATCC 30-2020 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++): ATCC 30-2101 Cell culture tested DMSO: ATCC 4-X
References:	2154: Spengler BA , et al.. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells established in vitro. In Vitro 8: 410, 1973. 22539: Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 26318: Seeger RC , et al. Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37: 1364-1371, 1977. PubMed: 856461 32287: Rostomily RC , et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 32469: Gromeier M , et al. Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proc. Natl. Acad. Sci. USA 93: 2370-2375, 1996. PubMed:

炫乐彩票

SK-N-MC(神经上皮ξ　瘤细胞)

炫乐彩票

SK-N-MC(神经上皮ξ 瘤细胞)

SK-N-MC(神经上皮ξ　瘤细胞)