LS 174T(结肠腺癌细』胞)_教育☉装备采购网

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品　　牌：ATCC

产品型号：CL-188 (株)

制造厂商：上海锐聪科技发展有限公【司

所在地区：上海

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LS 174T(结肠腺癌细胞)

LS 174T(结肠腺癌细胞)

Cell Biology
ATCC^® Number:	CL-188™	Price:	$203.00
Designations:	LS 174T	Depositors:	Northwestern University
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	epithelial

Source:	Organ: colon Disease: colorectal adenocarcinoma Tumor stage: Dukes type B
Cellular Products:	carcinoembryonic antigen (CEA)(The production of CEA in the ATCC seed stock was 1944 ng per 10(6) cells in 10 days.) interleukin 10 (IL-10) [22511] interleukin 6 (IL-6) mucin
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
This material is cited in a U.S. and/or other Patent or Patent Application, and may not be used to infringe on the patent claims.
Related Cell Culture Products
Isolation:	(The cell line was established from a Dukes type B adenocarcinoma of the colon. The tissue was minced and cultured without transfer for 10 months.) [2148]
Tumorigenic:	Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Reverse Transcript:	negative
Oncogene:	myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src -
Antigen Expression:	serologically defined colon cancer antigen 3; Homo sapiens, expressed HLA A2, B13, B50; Blood type O
Cytogenetic Analysis:	45,X; one X chromosome missing; no other chromosomal aberrations
Isoenzymes:	ADA, 1; ES-D, 1; G6PD, B; PEP-D, 1; PGD, A; PGM1, 1; PGM3, 2
Age:	58 years
Gender:	female
Ethnicity:	Caucasian
Comments:	LS 174T is a variant of LS 180 (ATCC CL-187) that has been maintained by using trypsin in the subculture protocol. It is more easily subcultivated than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen (CEA). Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles [Pubmed ID: ]. They are negative for p53 antigen expression, but positive for mRNA expression. LS 174T cells stain positively for cytokeratins. The line is positive for expression of c-myc, N-myc, H-ras, N-ras. Myb, and fos oncogenes. K-ras and sis oncogene expression were not detected.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium renewal: 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003 recommended serum: ATCC 30-2020 derived from same individual: ATCC CL-187
References:	2148: Tom BH , et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 3524: Jakstys MM , et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980 22147: Chen TR , et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 22511: Gastl GA , et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 22861: Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 32265: Tsao H , et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 32377: Shibata D , et al. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat. Genet. 6: 273-281, 1994. PubMed: 32794: Kutchera W , et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed:

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LS 174T(结肠腺癌细胞)