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                教育装备采购网首页 > 产品库 > 产品≡分类大全 > 实验室设备 > 实验室耗材 > 其它
                
        
                
            
                
                
                A172(胶质母细胞№瘤细胞)
                
                
                
                    
                
                        
                
                            
                A172(胶◎质母细胞瘤细胞)
                
                            
                
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                ATCC
                
                            
                CRL-1620 (株)
                
                            
                上海锐聪』科技发展有限公司
                
                            
                            
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                A172(胶质母细胞瘤细胞)
                            
                                        
                A172(胶质母细胞瘤细胞) 
                                            		        
                        
                                     
                        
                                        
                                                    
                                                                
                Cell Biology
                                                        		                    
                                    
                                        
                                            ATCC® Number:                            CRL-1620™                                 Price:                            $203.00                        
                                                        
                                                                
                Designations:
                                                        		                                                
                A-172 [A172]
                                                        		                                                
                Depositors:
                                                        		                                                
                DJ Giard
                                                        		                    
                                    
                                                                
                Biosafety Level:
                                                        		                                                
                1
                                                        		                                                
                Shipped:
                                                        		                                                
                frozen
                                                        		                    
                                    
                                                                
                Medium & Serum:
                                                        		                                                
                See Propagation
                                                        		                                                
                Growth Properties:
                                                        		                                                
                adherent
                                                        		                    
                                    
                                                                
                Organism:
                                                        		                                                
                Homo sapiens (human)
                                                        		                                                                      
                                    
                                                             
                                    
                                        Source:                                                
                Organ: brain
                                        Disease: glioblastoma
                                                        		                    
                                    
                                        Permits/Forms:                                                
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                                                        		                    
                                    
                                                            
                                    
                                        Tumorigenic:                                                
                Yes, form colonies in semisolid medium.
                                        No, the cells were not tumorigenic in immunosuppressed mice
                                                        		                    
                                    
                                        DNA Profile
                                        (STR):                		                                                
                Amelogenin: X,Y
                                        CSF1PO: 9,12
                                        D13S317: 11
                                        D16S539: 12
                                        D5S818: 11,12
                                        D7S820: 11
                                        THO1: 6,9.3
                                        TPOX: 8,11
                                        vWA: 20
                                                        		                    
                                    
                                        Age:                                                
                53 years
                                                        		                    
                                    
                                        Gender:                                                
                male
                                                        		                    
                                    
                                        Propagation:                                                
                ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                                        Temperature: 37.0C
                                                        		                    
                                    
                                        Subculturing:                                                
                Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
                                        
                                        Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
                                        
                                        Medium renewal: Every 2 to 3 days
                                                        		                    
                                    
                                        Preservation:                                                
                Freeze medium: culture medium 95%; DMSO, 5%
                                        Storage temperature: liquid nitrogen vapor phase
                                                        		                    
                                    
                                        Related Products:                                                
                Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002
                                        recommended serum: ATCC 30-2020
                                                        		                    
                                    
                                        References:                                                
                23094: Olopade OI , et al. Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 
                                        23218: Giard DJ , et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 
                                        32550: Debinski W , et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 
                                                        		                    
                                                            
                                            		        
                    
                 
                
                
                
                
                
                
                    
                
                        
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