HuT78(T淋巴细胞白★血病细胞)

产品报价：面议

留言咨询

产品热度：加载中

品　　牌：ATCC

产品型号：TIB-161 (株)

制造厂商：上海锐聪科技发∩展有限公司

所在地区：上海

详细说明

HuT78(T淋巴细胞白血病细胞)

HuT78(T淋巴细胞白血病细胞)

Cell Biology
ATCC^® Number:	TIB-161™	Price:	$203.00
Designations:	HuT 78	Depositors:	AF Gazdar
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	suspension
Organism:	Homo sapiens (human)	Morphology:	lymphoblast

Source:	Cell type: cutaneous T lymphocyte Disease: lymphoma, cutaneous T cell
Cellular Products:	interleukin 2 [1140]
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Isolation:	(Hut 78 was derived from peripheral blood of a patient with Sezary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2, and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for Hut 78.)
Applications:	transfection host (technology from amaxa)
Receptors:	interleukin 2 (IL-2), expressed [1140]
Tumorigenic:	Yes, in nude mice when injected intracranially
Antigen Expression:	CD4; Homo sapiens [22610]
DNA Profile (STR):	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 11,12 D5S818: 11,12 D7S820: 8,11 THO1: 8,9 TPOX: 8,9 vWA: 14,15
Age:	53 years adult
Gender:	male
Ethnicity:	Caucasian
Comments:	H9 (ATCC HTB-176) is a clonal derivative of HuT 78 [Pubmed: ].
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. Temperature: 37.0C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing:	Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp4 and 8 X 10 exp5 viable cells/ml. maintain cell density at less than 1 X 10 exp6 cells/ml. Medium renewal: Two to three times weekly
Preservation:	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Doubling Time:	about 65 hours
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2005 recommended serum: ATCC 30-2020 derivative: ATCC HTB-176
References:	1140: Gootenberg JE , et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 22484: Mann DL , et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 22610: Gazdar AF , et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 23228: Chen TR . Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 23420: OConnell MA , et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 32283: Hu SX , et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 32396: Kolanus W , et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 32796: Bloom TJ , Beavo JA . Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed:

手机彩票网

HuT78(T淋巴细胞白血病细胞)