LLC-MK2(恒河猴肾细№胞)_教育装备采▅购网

LLC-MK2(恒河猴肾细胞)

产品报价：面议

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产品热度：加载中

品　　牌：ATCC

产品型号：CCL-7 (株)

制造厂商：上海锐聪科技发展有限公司

所在地区：上海

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LLC-MK2(恒河猴肾细胞)

LLC-MK2(恒河猴肾细胞)

Cell Biology
ATCC^® Number:	CCL-7™	Price:	$255.00
Designations:	LLC-MK2 Original	Depositors:	RN Hull
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Macaca mulatta (monkey, rhesus)	Morphology:	epithelial

Source:	Organ: kidney Disease: normal
Cellular Products:	plasminogen activator
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Virus Susceptibility:	poliovirus 1; coxsackievirus B4; bovine viral diarrhea (BVD) virus
Reverse Transcript:	negative
Age:	adult
Comments:	The serum used in the medium should be pre-tested for good quality. Some lots of horse serum do support satisfactory growth. Adaptation to other types of sera generally results in slow growth with a prolonged lag in growth.
Propagation:	ATCC complete growth medium: Medium 199 (with 1.68 g/L bicarbonate), 99%; horse serum, 1% Temperature: 37.0C
Subculturing:	Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:10 is recommended Medium renewal: Every 2 to 3 days
Preservation:	Culture medium, 95%; DMSO, 5%
Related Products:	recommended serum: ATCC 30-2040
References:	22310: Anat. Rec. 124: 490, 1956. 26049: Hull RN , et al. Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research. J. Exp. Med. 115: 903-918, 1962. PubMed: 26065: Hull RN , Tritch OJ . Characterization of cell strains by viral susceptibility. Natl. Cancer Inst. Monogr. 7: 161-172, 1962. PubMed:

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