每日彩票

  • <tr id='aWJqVw'><strong id='aWJqVw'></strong><small id='aWJqVw'></small><button id='aWJqVw'></button><li id='aWJqVw'><noscript id='aWJqVw'><big id='aWJqVw'></big><dt id='aWJqVw'></dt></noscript></li></tr><ol id='aWJqVw'><option id='aWJqVw'><table id='aWJqVw'><blockquote id='aWJqVw'><tbody id='aWJqVw'></tbody></blockquote></table></option></ol><u id='aWJqVw'></u><kbd id='aWJqVw'><kbd id='aWJqVw'></kbd></kbd>

    <code id='aWJqVw'><strong id='aWJqVw'></strong></code>

    <fieldset id='aWJqVw'></fieldset>
          <span id='aWJqVw'></span>

              <ins id='aWJqVw'></ins>
              <acronym id='aWJqVw'><em id='aWJqVw'></em><td id='aWJqVw'><div id='aWJqVw'></div></td></acronym><address id='aWJqVw'><big id='aWJqVw'><big id='aWJqVw'></big><legend id='aWJqVw'></legend></big></address>

              <i id='aWJqVw'><div id='aWJqVw'><ins id='aWJqVw'></ins></div></i>
              <i id='aWJqVw'></i>
            1. <dl id='aWJqVw'></dl>
              1. <blockquote id='aWJqVw'><q id='aWJqVw'><noscript id='aWJqVw'></noscript><dt id='aWJqVw'></dt></q></blockquote><noframes id='aWJqVw'><i id='aWJqVw'></i>
                教育装备采购网
                长春智慧学々校体育论坛1180*60
                教育装备展示厅
                www.freecchost.com
                教育装备采购网首页 > 产品库 > 产□品分类大全 > 实验室设备 > 实验室耗材 > 其它

                293 [HEK-293] (人胚↓肾细胞)

                293 [HEK-293] (人胚肾细胞)
                <
                • 293 [HEK-293] (人胚肾细胞)
                >
                产品报价: 面议
                留言咨询
                加载中
                ATCC
                CRL-1573 (株)
                上海锐聪』科技发展有限公司
                上海
                详细说明

                293 [HEK-293] (人胚肾细胞)

                293 [HEK-293] (人胚肾细胞)
                 
                Cell Biology
                ATCC® Number: CRL-1573™ Price: $203.00
                Designations:
                293 [HEK-293]
                Depositors:
                FL Graham
                2 [CELLS CONTAIN ADENOVIRUS]
                Shipped:
                frozen
                Medium & Serum:
                Growth Properties:
                adherent
                Organism:
                Homo sapiens (human)
                Morphology:
                epithelial
                 
                Source:
                Organ: kidney
                Permits/Forms:
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                Restrictions:
                These cells are distributed for research purposes only. 293 cells, their products, or their derivatives may not be distributed to third parties.
                Applications:
                efficacy testing [92587]
                transfection host (technology from amaxa
                Roche FuGENE® Transfection Reagents)
                viruscide testing [92579]
                Receptors:
                vitronectin, expressed
                Tumorigenic:
                Yes, forms tumors in nude mice
                DNA Profile
                (STR):
                Amelogenin: X
                CSF1PO: 11,12
                D13S317: 12,14
                D16S539: 9,13
                D5S818: 8,9
                D7S820: 11,12
                THO1: 7,9.3
                TPOX: 11
                vWA: 16,19
                Cytogenetic
                Analysis:
                This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2 %. The der(1)t(1;15) (q42;q13), der(19)t(3;19) (q12;q13), der(12)t(8;12) (q22;p13), and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+, and a single Xp+ in most cells.
                Age:
                fetus
                Comments:
                Although an earlier report suggested that the cells contained Adenovirus 5 DNA from both the right and left ends of the viral genome [RF32764], it is now clear that only left end sequences are present. [39768]
                The line is excellent for titrating human adenoviruses.
                The cells express an unusual cell surface receptor for vitronectin composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit. [23406]
                The Ad5 insert was cloned and sequenced, and it was determined that a colinear seqment from nts 1 to 4344 is integrated into chromosome 19 (19q13.2). [39768]
                Propagation:
                ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated horse serum to a final concentration of 10%.
                Temperature: 37.0C
                Atmosphere: air, 95%; carbon dioxide (CO2), 5%
                Growth Conditions: The cell line does not adhere to the substrate when left at room temperature for any length of time, therefore, live cultures may be received with the cells detached.The cells will re-attach to the flask over a period of several days in culture at 37C.
                Subculturing:
                Protocol:
                1. Remove and discard culture medium.
                2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
                3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
                  Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
                4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
                5. Add appropriate aliquots of the cell suspension to new culture vessels.
                6. Incubate cultures at 37°C.


                Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

                Medium renewal: Every 2 to 3 days
                Preservation:
                Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
                Storage temperature: liquid nitrogen vapor phase
                Related Products:
                Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003
                derivative: ATCC CRL-10852
                derivative: ATCC CRL-12006
                derivative: ATCC CRL-12007
                derivative: ATCC CRL-12013
                derivative: ATCC CRL-12479
                derivative: ATCC CRL-2029
                derivative: ATCC CRL-2368
                purified DNA: ATCC CRL-1573D
                References:
                21624: Xie QW , et al. Complementation analysis of mutants of nitric oxide synthase reveals that the active site requires two hemes. Proc. Natl. Acad. Sci. USA 93: 4891-4896, 1996. PubMed:
                21631: Da Costa LT , et al. Converting cancer genes into killer genes. Proc. Natl. Acad. Sci. USA 93: 4192-4196, 1996. PubMed:
                22282: Graham FL , et al. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-72, 1977. PubMed: 886304
                22319: Graham FL , et al. Defective transforming capacity of adenovirus type 5 host-range mutants. Virology 86: 10-21, 1978. PubMed: 664220
                22699: Harrison T , et al. Host-range mutants of adenovirus type 5 defective for growth in HeLa cells. Virology 77: 319-329, 1977. PubMed: 841862
                23406: Bodary SC , McLean JW . The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line. J. Biol. Chem. 265: 5938-5941, 1990. PubMed:
                27819: Goodrum FD , Ornelles DA . The early region 1B 55-kilodalton oncoprotein of adenovirus relieves growth restrictions imposed on viral replication by the cell cycle. J. Virol. 71: 548-561, 1997. PubMed:
                28301: Loffler S , et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed:
                32283: Hu SX , et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed:
                32396: Kolanus W , et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed:
                32490: Stauderman KA , et al. Characterization of human recombinant neuronal nicotinic acetylcholine receptor subunit combinations alpha 2 beta 4, alpha 3 beta 4 and alpha 4 beta 4 stably expressed in HEK293 cells. J. Pharmacol. Exp. Ther. 284: 777-789, 1998. PubMed:
                32514: Bartz SR , et al. Human immunodeficiency virus type 1 cell cycle control: Vpr is cytostatic and mediates G2 accumulation by a mechanism which differs from DNA damage checkpoint control. J. Virol. 70: 2324-2331, 1996. PubMed:
                32726: Sandri-Goldin RM , Hibbard MK . The herpes simplex virus type 1 regulatory protein ICP27 coimmunoprecipitates with anti-sm antiserum, and the C terminus appears to be required for this interaction. J. Virol. 70: 108-118, 1996. PubMed:
                32829: Ansieau S , et al. Tumor necrosis factor receptor-associated factor (TRAF)-1, TRAF-2, and TRAF-3 interact in vivo with the CD30 cytoplasmic domain; TRAF-2 mediates CD30-induced nuclear factor kappa B activation. Proc. Natl. Acad. Sci. USA 93: 14053-14058, 1996. PubMed:
                32893: Zhang J , et al. Dynamin and beta-arrestin reveal distinct mechanisms for G protein-coupled receptor internalization. J. Biol. Chem. 271: 18302-18305, 1996. PubMed:
                32914: Oppermann M , et al. Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases. Proc. Natl. Acad. Sci. USA 93: 7649-7654, 1996. PubMed:
                32921: Xia Y , et al. Nitric oxide synthase generates superoxide and nitric oxide in arginine-depleted cells leading to peroxynitrite-mediated cellular injury. Proc. Natl. Acad. Sci. USA 93: 6770-6774, 1996. PubMed:
                32925: Zhu X , et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed:
                32971: Uebele VN , et al. Functional differences in Kv1.5 currents expressed in mammalian cell lines are due to the presence of endogenous Kvbeta2.1 subunits. J. Biol. Chem. 271: 2406-2412, 1996. PubMed:
                33003: Abell A , et al. Deletions of portions of the extracellular loops of the lutropin/choriogonadotropin receptor decrease the binding affinity for ovine luteinizing hormone, but not human choriogonadotropin, by preventing the formation of mature cell surface receptor. J. Biol. Chem. 271: 4518-4527, 1996. PubMed:
                33010: Tiberi M , et al. Differential regulation of dopamine D1A receptor responsiveness by various G protein-coupled receptor kinases. J. Biol. Chem. 271: 3771-3778, 1996. PubMed:
                33022: Shahrestanifar M , et al. Studies on inhibition of mu and delta opioid receptor binding by dithiothreitol and N-ethylmaleimide. His223 is critical for mu opioid receptor binding and inactivation by N-ethylmaleimide. J. Biol. Chem. 271: 5505-5512, 1996. PubMed:
                33035: Boring L , et al. Molecular cloning and functional expression of murine JE (monocyte chemoattractant protein 1) and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed:
                33036: Noonberg SB , et al. Evidence of post-transcriptional regulation of U6 small nuclear RNA. J. Biol. Chem. 271: 10477-10481, 1996. PubMed:
                33050: Fox JC , Shanley JR . Antisense inhibition of basic fibroblast growth factor induces apoptosis in vascular smooth muscle cells. J. Biol. Chem. 271: 12578-12584, 1996. PubMed:
                33056: Lee MJ , et al. The inducible G protein-coupled receptor edg-1 signals via the Gi/mitogen-activated protein kinase pathway. J. Biol. Chem. 271: 11272-11279, 1996. PubMed:
                33123: Marchand P , et al. Cysteine mutations in the MAM domain result in monomeric meprin and alter stability and activity of the proteinase. J. Biol. Chem. 271: 24236-24241, 1996. PubMed:
                33137: Arai H , Charo IF . Differential regulation of G-protein-mediated signaling by chemokine receptors. J. Biol. Chem. 271: 21814-21819, 1996. PubMed:
                33138: Huang Q , et al. Substrate recognition by tissue factor-factor VIIa. J. Biol. Chem. 271: 21752-21757, 1996. PubMed:
                33157: Monteclaro FS , Charo IF . The amino-terminal extracellular domain of the MCP-1 receptor, but not the RANTES/MIP-1alpha receptor, confers chemokine selectivity. J. Biol. Chem. 271: 19084-19092, 1996. PubMed:
                33158: Keith DE , et al. Morphine activates opioid receptors without causing their rapid internalization. J. Biol. Chem. 271: 19021-19024, 1996. PubMed:
                39768: Louis N , et al. Cloning and sequencing of the cellular-viral junctions from the human adenovirus type 5 transformed 293 cell line. Virology 233: 423-429, 1997. PubMed:
                61259: Shaw G , et al. Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 16: 869-871, 2002. PubMed:
                92579: Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Fingerpads of Adult Volunteers. West Conshohocken, PA: ASTM International; ASTM Standard Test Method E 1838-02.
                92587: Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides. West Conshohocken, PA: ASTM International; ASTM Standard Test Method E 2197-02.

                 

                留言咨询
                姓名
                电话
                单位
                信箱
                留言内容
                提交留言
                联系我时,请说明◥是在教育装备采购网上看到的,谢谢!
                同类产品推荐