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                教育◣装备采购网首页 > 产品库 > 产品分类大全 > 实验室设备 > 实验室耗▓材 > 其它
                
        
                
            
                
                
                R 1610(中国№仓鼠体细胞)
                
                
                
                    
                
                        
                
                            
                R 1610(中国◤仓鼠体细胞)
                
                            
                
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                ATCC
                
                            
                CRL-1657 (株)
                
                            
                上海锐聪科技发展有限公司
                
                            
                            
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                详细说明
                
                    
                R 1610(中国仓鼠】体细胞)
                            
                                        
                R 1610(中国仓鼠体细胞) 
                                            		        
                        
                                     
                        
                                        
                                                    
                                                                
                Cell Biology
                                                        		                    
                                    
                                        
                                            ATCC® Number:                            CRL-1657™                                 Price:                            $330.00                        
                                                        
                                                                
                Designations:
                                                        		                                                
                R 1610
                                                        		                                                
                Depositors:
                                                        		                                                
                M Rosenberg
                                                        		                    
                                    
                                                                
                Biosafety Level:
                                                        		                                                
                1
                                                        		                                                
                Shipped:
                                                        		                                                
                frozen
                                                        		                    
                                    
                                                                
                Medium & Serum:
                                                        		                                                
                See Propagation
                                                        		                                                
                Growth Properties:
                                                        		                                                
                adherent
                                                        		                    
                                    
                                                                
                Organism:
                                                        		                                                
                Cricetulus griseus (hamster, Chinese)
                                                        		                                                
                Morphology:
                                                        		                                                
                fibroblast
                                                        		                    
                                    
                                                             
                                    
                                        Source:                                                
                Organ: lung
                                                        		                    
                                    
                                        Permits/Forms:                                                
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                                                        		                    
                                    
                                                            
                                    
                                        Applications:                                                
                transfection host
                                                        		                    
                                    
                                        Gender:                                                
                male
                                                        		                    
                                    
                                        Comments:                                                
                This line is a clonal derivative of V79 cells (see also V79-4, ATCC CCL-93) that had been treated with ethylmethanesulfonate.
                                        The cells are deficient in galactokinase and are resistant to 2-deoxygalactose and 8-azaguanine.
                                                        		                    
                                    
                                        Propagation:                                                
                ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                                        Temperature: 37.0C
                                                        		                    
                                    
                                        Subculturing:                                                
                Protocol:                        
                                              
                1. Remove and discard culture medium.
                  2.                             
                2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
                  3.                             
                3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
                                              Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
                  4.                             
                4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
                  5.                             
                5. Add appropriate aliquots of the cell suspension to new culture vessels.
                  6.                             
                6. Incubate cultures at 37°C.
                  7.                         
                                        
                                        
                                        Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
                                        
                                        Medium renewal: 2 to 3 times per week
                                                        		                    
                                    
                                        Preservation:                                                
                Freeze medium: Complete growth medium 95%; DMSO, 5%
                                        Storage temperature: liqid nitrogen vapor phase
                                                        		                    
                                    
                                        Related Products:                                                
                Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002
                                        recommended serum: ATCC 30-2020
                                        parental cell line: ATCC CCL-93
                                                        		                    
                                    
                                        References:                                                
                10111: Schumperli D , et al. Efficient expression of Escherichia coli galactokinase gene in mammalian cells. Proc. Natl. Acad. Sci. USA 79: 257-261, 1982. PubMed: 
                                        25665: Thirion JP , et al. Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose. Genetics 83: 137-147, 1976. PubMed: 
                                                        		                    
                                                            
                                            		        
                    
                 
                
                
                
                
                
                
                    
                
                        
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