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Cell Biology
ATCC® Number:	HTB-81™	Price:	$203.00
Designations:	DU 145	Depositors:	KR Stone
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	epithelial
Source:	Organ: prostate Disease: carcinoma Derived from metastatic site: brain
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Isolation:	(DU 145 was isolated by K.R. Stone et al from a lesion in the brain of a patient with metastatic carcinoma of the prostate and a 3 year history of lymphocytic leukemia.)
Applications:	transfection host (technology from amaxa Roche FuGENE® Transfection Reagents)
Tumorigenic:	Yes, in nude mice; forms adenocarcinoma (grade II) consistent with prostatic primary
Antigen Expression:	Blood Type O; Rh+
Cytogenetic Analysis:	This is a hypotriploid human cell line. Both 61 and 62 chromosome numbers had the highest rate of occurrence in 30 metaphase counts.The rate of higher ploidies was 3%. The t(11q12q), del(11)(q23), 16q+, del(9)(p11), del(1)(p32) and 6 other marker chromosomes were found in most cells. The N13 was usually absent. The Y chromosome is abnormal through translocation to an unidentified chromosomal segment. The X chromosome was present in single copy.
Isoenzymes:	AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 1-2; PGM1, 1; PGM3, 2
Age:	69 years
Gender:	male
Ethnicity:	Caucasian
Comments:	The line is not detectably hormone sensitive, is only weakly positive for acid phosphatase and isolated cells form colonies in soft agar. The cells do not express prostate antigen. Ultrastructural analyses of both the cell line and original tumor revealed microvilli, tonofilaments, desmosomes, any mitochondria, well developed Golgi and heterogenous lysosomes.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37C. Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium renewal: 2 to 3 times per week
Preservation:	Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liqid nitrogen vapor temperature
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003 recommended serum: ATCC 30-2020 purified DNA: ATCC HTB-81D 0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++): ATCC 30-2101 Cell culture tested DMSO: ATCC 4-X
References:	22289: Papsidero LD , et al. Prostate antigen: a marker for human prostate epithelial cells. J. Natl. Cancer Inst. 66: 37-42, 1981. PubMed: 22858: Stone KR , et al. Isolation of a human prostate carcinoma cell line (DU 145). Int. J. Cancer 21: 274-281, 1978. PubMed: 631930 23028: Mickey DD , et al. Heterotransplantation of a human prostatic adenocarcinoma cell line in nude mice. Cancer Res. 37: 4049-4058, 1977. PubMed: 908039 23226: Pollack MS , et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 32283: Hu SX , et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 32341: Sheng S , et al. Maspin acts at the cell membrane to inhibit invasion and motility of mammary and prostatic cancer cells. Proc. Natl. Acad. Sci. USA 93: 11669-11674, 1996. PubMed: 32460: Carter RE , et al. Prostate-specific membrane antigen is a hydrolase with substrate and pharmacologic characteristics of a neuropeptidase. Proc. Natl. Acad. Sci. USA 93: 749-753, 1996. PubMed: 32486: Nupponen NN , et al. Genetic alterations in prostate cancer cell lines detected by comparative genomic hybridization. Cancer Genet. Cytogenet. 101: 53-57, 1998. PubMed: 32768: Robinson D , et al. A tyrosine kinase profile of prostate carcinoma. Proc. Natl. Acad. Sci. USA 93: 5958-5962, 1996. PubMed: 32916: Su ZZ , et al. Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family. Proc. Natl. Acad. Sci. USA 93: 7252-7257, 1996. PubMed: 32925: Zhu X , et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 33090: Boffa LC , et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996. PubMed: