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                YAC-1(淋巴瘤细胞)

                YAC-1(淋巴瘤细胞)
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                • YAC-1(淋巴瘤细胞)
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                ATCC
                TIB-160 (株)
                上海锐聪科技发展有限公司
                上海
                详细说明

                YAC-1(淋巴瘤细胞)

                YAC-1(淋巴瘤细胞)
                 
                Cell Biology
                ATCC® Number: TIB-160™ Price: $203.00
                Designations:
                YAC-1
                Depositors:
                JY Djeu
                1
                Shipped:
                frozen
                Medium & Serum:
                Growth Properties:
                suspension
                Organism:
                Mus musculus (mouse)
                Morphology:
                lymphoblast
                 
                Source:
                Disease: lymphoma
                Permits/Forms:
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                Isolation:
                (YAC-1 is a lymphoma which was induced by inoculation of the Moloney leukemia virus (MLV) into a newborn A/Sn mouse.) [22345]
                Applications:
                assay of NK cell activity [22345] [49299] (The cells are sensitive to the action of natural killer (NK) cells and are useful in assays of NK cell activity.)
                Strain:
                A/Sn
                Comments:
                Tested and found negative for ectromelia virus (mousepox).
                Propagation:
                ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                Temperature: 37.0C
                Atmosphere: air, 95%; carbon dioxide (CO2), 5%
                Subculturing:
                Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 10(5) cells/ml and maintain between 2 X 10(5) and 2 X 10(6) cells/ml.

                Medium renewal: Every 2 to 3 days
                Preservation:
                Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
                Storage temperature: liquid nitrogen vapor phase
                Related Products:
                Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2001
                recommended serum: ATCC 30-2020
                Cell culture tested DMSO: ATCC 4-X
                References:
                22345: Kiessling R , et al. "Natural" killer cells in the mouse. I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype. Eur. J. Immunol. 5: 112-117, 1975. PubMed:
                26348: Cikes M , Friberg S Jr. Progressive loss of H-2 antigens with concomitant increase of cell- surface antigen(s) determined by Moloney leukemia virus in cultured murine lymphomas. J. Natl. Cancer Inst. 50: 347-362, 1973. PubMed:
                32552: Okazaki IJ , et al. Cloning and characterization of a novel membrane-associated lymphocyte NAD:arginine ADP-ribosyltransferase. J. Biol. Chem. 271: 22052-22057, 1996. PubMed:
                49299: Kiessling R , et al. "Natural" killer cells in the mouse. II. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Characteristics of the killer cell. Eur. J. Immunol. 5: 117-121, 1975. PubMed:
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