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                P3X63Ag8(骨髓「瘤细胞)

                P3X63Ag8(骨髓瘤细胞)
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                • P3X63Ag8(骨髓瘤细胞)
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                ATCC
                TIB-9 (株)
                上海锐聪科技发展有限公ω司
                上海
                详细说明

                P3X63Ag8(骨髓瘤细胞)

                P3X63Ag8(骨髓瘤细胞)
                 
                Cell Biology
                ATCC® Number: TIB-9™ Price: $330.00
                Designations:
                P3X63Ag8
                Depositors:
                G Kohler
                C Milstein
                Isotype:
                IgG1; kappa light chain
                1
                Shipped:
                frozen
                Medium & Serum:
                Growth Properties:
                suspension
                Organism:
                Mus musculus (mouse)
                Morphology:
                lymphoblast
                 
                Source:
                Cell type: B lymphocyte
                Disease: plasmacytoma; myeloma
                Cellular Products:
                immunoglobulin; monoclonal antibody
                Permits/Forms:
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                Antigen Expression:
                H-2d
                Cytogenetic
                Analysis:
                hyperdiploid; modal number = 65
                Strain:
                BALB/c
                Comments:
                This line was derived from the P3K cell line (a tissue culture line established from the MOPC-21 plasmacytoma).
                The line is resistant to 0.1 mM 8-azaguanine and die in HAT medium.
                The cells have been reported to be cholesterol auxotrophs due to a deficiency in 3-ketosteroid reductase activity.
                Tested and found negative for ectromelia virus (mousepox).
                Propagation:
                ATCC complete growth medium: Dulbeccos modified Eagles medium with 4.5 g/L glucose, 90%; fetal bovine serum, 10%
                Subculturing:
                Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
                Medium renewal: Every 2 to 3 days
                References:
                1042: Koprowski H , et al. Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proc. Natl. Acad. Sci. USA 74: 2985-2988, 1977. PubMed: 268647
                1112: Kohler G , Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256: 495-497, 1975. PubMed:
                22154: Horibata K , Harris AW . Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed:
                22173: Kohler G , et al. Fusion of T and B cells. Somatic Cell Genet. 3: 303-312, 1977. PubMed: 305123
                22347: Kohler G , Milstein C . Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur. J. Immunol. 6: 511-519, 1976. PubMed: 825377
                23366: Sato JD , et al. Effects of proximate cholesterol precursors and steroid hormones on mouse myeloma growth in serum-free medium. In Vitro Cell. Dev. Biol. 24: 1223-1228, 1988. PubMed:
                26333: Ramasamy R , et al. Possible role for the Fc receptor on B lymphocytes. Nature 249: 573-574, 1974. PubMed:
                26334: Lemke H , et al. Hybrid cell lines secreting monoclonal antibody specific for major histocompatibility antigens of the mouse. Nature 271: 249-251, 1978. PubMed: 74805
                26335: Eur. J. Immunol. 3: 135-140, 1973.
                26336: Secher DS , et al. Spontaneous mutation in tissue culture-chemical nature of variant immunoglobulin from mutant clones of MOPC 21. FEBS Lett. 37: 311-316, 1973. PubMed:

                 

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