EL4(淋巴瘤▃细胞)_教育☉装备采购网

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品　　牌：ATCC

产品型号：TIB-39 (株)

制造厂商：上海锐聪科技发展有限公司

所在地区：上海

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EL4(淋巴瘤细胞)

EL4(淋巴瘤细胞)

Cell Biology
ATCC^® Number:	TIB-39™	Price:	$203.00
Designations:	EL4	Depositors:	M Cohn
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	suspension
Organism:	Mus musculus (mouse)	Morphology:	lymphoblast

Source:	Cell type: T lymphocyte Disease: lymphoma
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Applications:	transfection host (technology from amaxa Roche FuGENE® Transfection Reagents)
Antigen Expression:	H-2b; Thy-1.2
Cytogenetic Analysis:	modal number = 39
Strain:	C57BL/6N
Comments:	EL4 was established from a lymphoma induced in a C57BL mouse by 9,10-dimethyl-1,2-benzanthracene. [22448] The cells are resistant to 0.1 mM cortisol and sensitive to 20 mcg/ml PHA. A subline (EL4.IL-2, ATCC TIB-181) that produces high levels of interleukin-2 (IL-2, interleukin 2) is available. Tested and found negative for ectromelia virus (mousepox).
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%. Temperature: 37.0C
Subculturing:	Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium renewal: Every 2 to 3 days
Preservation:	culture medium 95%; DMSO, 5%
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002 derivative: ATCC CRL-2113 derivative: ATCC TIB-181 derivative: ATCC TIB-40
References:	1104: Ralph P . Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 22422: Old LJ , et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 22448: Gorer PA . Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 22523: Herberman RB . Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 22528: Ralph P , Nakoinz I . Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 23443: Shevach EM , et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 32256: Aparicio CL , et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 32286: Cuthbert JA , Lipsky PE . Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed:

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EL4(淋巴瘤细胞)