SW480 [SW-480]（人结ω　肠癌细胞）

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品　　牌：ATCC

产品型号：CCL-228 (株)

制造厂商：上海锐聪科技发展有限公司

所在地区：上海

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SW480 [SW-480]（人结肠癌细胞）

SW480 [SW-480]（人结肠癌细胞）

Cell Biology
ATCC^® Number:	CCL-228™	Price:	$203.00
Designations:	SW480 [SW-480]	Depositors:	A Leibovitz
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	epithelial

Source:	Organ: colon Disease: colorectal adenocarcinoma Tumor stage: Dukes type B
Cellular Products:	carcinoembryonic antigen (CEA) 0.7 ng/10 exp6 cells/10 days; keratin; transforming growth factor beta
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Related Cell Culture Products
Applications:	transfection host (technology from amaxa Roche FuGENE® Transfection Reagents)
Receptors:	epidermal growth factor (EGF)
Virus Susceptibility:	Human immunodeficiency virus 1
Tumorigenic:	Yes, in nude mice [22539] (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells)
Reverse Transcript:	negative
Oncogene:	myc +; myb + ; ras +; fos +; sis +; p53 +; abl -; ros -; src -
Antigen Expression:	HLA A2, B8, B17; blood type A; Rh+
DNA Profile (STR):	Amelogenin: X CSF1PO: 13,14 D13S317: 12 D16S539: 13 D5S818: 13 D7S820: 8 THO1: 8 TPOX: 11 vWA: 16
Cytogenetic Analysis:	The stemline chromosome number is hypotriploid and 11-12 marker chromosomes were common. Both double minutes and dicentrics were observed in 8% of each metaphase examined.
Isoenzymes:	ES-D, 1; G6PD, B; PEP-D, 1; PGD, A; PGM1, 2; PGM3, 1
Age:	50 years
Gender:	male
Ethnicity:	Caucasian
Comments:	SW480 was established from a primary adenocarcinoma of the colon. [23025] A cell line established from a lymph node metastasis taken from the same patient one year later is available (see ATCC CCL-227). [23025] The line is negative for CSAp (CSAp-) and colon antigen 3. The cells are positive for keratin by immunoperoxidase staining. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. [22870] [23322] The cells express elevated levels of p53 protein. [23322] The line is positive for expression of c-myc, K-ras, H-ras, N-ras, myb, sis and fos oncogenes. [22861] N-myc oncogene expression was not detected. [22861] Matrilysin, a metalloproteinase associated with tumor invasiveness, is not expressed. [49853] The cells have been reported to produce GM-CSF. [23114] This line has a mutation in codon 12 of the ras protooncogene, and can be used as a positive control for PCR assays of mutation in this codon. A culture at passage 91 was submitted to the American Type Culture Collection by A. Leibovitz in November, 1978.
Propagation:	ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0C Atmosphere: air, 100%
Subculturing:	Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. without CO2. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:8 is recommended Medium renewal: 1 to 2 times per week
Preservation:	Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase
Related Products:	Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2008 recommended serum: ATCC 30-2020 derived from same individual: ATCC CCL-227
References:	22536: Fogh J , et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 22539: Fogh J , et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 22545: Lelbovitz A , et al. Detection and analysis of a glucose 6-phosphate dehydrogenase phenotype B cell line contamination. J. Natl. Cancer Inst. 63: 635-645, 1979. PubMed: 288927 22564: Adachi A , et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 22794: Schroy PC , et al. Detection of p21ras mutations in colorectal adenomas and carcinomas by enzyme-linked immunosorbent assay. Cancer 76: 201-209, 1995. PubMed: 22861: Trainer DL , et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 22870: Weiss J , et al. Mutation and expression of the p53 gene in malignant melanoma cell lines. Int. J. Cancer 54: 693-699, 1993. PubMed: 22930: Nigro JM , et al. Mutations in the p53 gene occur in diverse human tumour types. Nature 342: 705-707, 1989. PubMed: 22996: Barnett SW , et al. Characterization of human immunodeficiency virus type 1 strains recovered from the bowel of infected individuals. Virology 182: 802-809, 1991. PubMed: 23025: Leibovitz A , et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 23071: Geiser AG , et al. Suppression of tumorigenicity in human cell hybrids derived from cell lines expressing different activated ras oncogenes. Cancer Res. 49: 1572-1577, 1989. PubMed: 23114: Lahm H , et al. Secretion of bioactive granulocyte-macrophage colony-stimulating factor by human colorectal carcinoma cells. Cancer Res. 54: 3700-3702, 1994. PubMed: 23322: Rodrigues NR , et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 25093: Santoro IM , Groden J . Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 32265: Tsao H , et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 32925: Zhu X , et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 49853: Witty JP , et al. Modulation of matrilysin levels in colon carcinoma cell lines affects tumorigenicity in vivo. Cancer Res. 54: 4805-4812, 1994. PubMed:

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SW480 [SW-480]（人结肠癌细胞）