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                Hep G2(人▽肝癌细胞)

                Hep G2(人肝癌★细胞)
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                • Hep G2(人肝癌细胞)
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                ATCC
                HB-8065 (株)
                上海锐聪科技发展有限公司
                上海
                详细说明

                Hep G2(人肝癌细胞)

                Hep G2(人肝癌细胞)
                 
                Cell Biology
                ATCC® Number: HB-8065™ Price: $203.00
                Designations:
                Hep G2
                Depositors:
                Wistar Institute
                1
                Shipped:
                frozen
                Medium & Serum:
                   
                Organism:
                Homo sapiens (human)
                Morphology:
                epithelial
                Hep G2(人肝癌细胞)
                 
                Source:
                Organ: liver
                Disease: hepatocellular carcinoma
                Cellular Products:
                alpha-fetoprotein (alpha fetoprotein); albumin; alpha2 macroglobulin (alpha-2-macroglobulin); alpha1 antitrypsin (alpha-1-antitrypsin); transferrin; alpha1 antichymotrypsin; (alpha-1-antichymotrypsin); haptoglobin; ceruloplasmin; plasminogen; [3525],complement (C4); C3 activator; fibrinogen; alpha1 acid glycoprotein (alpha-1 acid glycoprotein); alpha2 HS glycoprotein (alpha-2-HS-glycoprotein); beta lipoprotein (beta-lipoprotein); retinol binding protein (retinol-binding protein) [3525]
                Permits/Forms:
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                This material is cited in a U.S. and/or other Patent or Patent Application, and may not be used to infringe on the patent claims.
                Applications:
                Receptors:
                insulin; insulin-like growth factor II (IGF II) [22446]
                Tumorigenic:
                No, in immunosuppressed mice
                Yes, in semisolid medium
                DNA Profile
                (STR):
                Amelogenin: X,Y
                CSF1PO: 10,11
                D13S317: 9,13
                D16S539: 12,13
                D5S818: 11,12
                D7S820: 10
                F13A01: 5,7
                F13B: 6,10
                FESFPS: 11
                LPL: 10,11
                THO1: 9
                TPOX: 8,9
                vWA: 17
                Cytogenetic
                Analysis:
                modal number = 55 (range = 50 to 60); has a rearranged chromosome 1
                Age:
                15 years adolescent
                Gender:
                male
                Ethnicity:
                Caucasian
                Comments:
                The cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities. [23557]
                The cells demonstrate decreased expression of apoA-I mRNA and increased expression of catalase mRNA in response to gramoxone (oxidative stress). [26594]
                There is no evidence of a Hepatitis B virus genome in this cell line. [1205] [22909]
                Propagation:
                ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                Temperature: 37.0C
                Subculturing:
                Protocol:
                1. Remove and discard culture medium.
                2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
                3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
                  Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
                4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
                5. Add appropriate aliquots of the cell suspension to new culture vessels.
                6. Incubate cultures at 37°C.


                    Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

                    Medium renewal: Twice per week
                Preservation:
                Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
                Storage temperature: liquid nitrogen vapor phase
                Related Products:
                Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2003
                recommended serum: ATCC 30-2020
                derivative: ATCC CRL-10741
                derivative: ATCC CRL-11997
                purified DNA: ATCC HB-8065D
                References:
                1205: Knowles BB , et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed:
                3525: Knowles BB , Aden DP . Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983
                22446: Schardt C , et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed:
                22909: Aden DP , et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137
                23557: Busch SJ , et al. Differential regulation of hepatic triglyceride lipase and 3-hydroxy-3- methylglutaryl-CoA reductase gene expression in a human hepatoma cell line, HepG2. J. Biol. Chem. 265: 22474-22479, 1990. PubMed:
                24388: Darlington GJ , et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed:
                26594: Cuthbert C , et al. Regulation of human apolipoprotein A-I gene expression by gramoxone. J. Biol. Chem. 272: 14954-14960, 1997. PubMed:
                27297: Deleersnyder V , et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed:
                32352: Benn J , et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed:
                32373: Goodrum FD , et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed:
                32396: Kolanus W , et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed:
                32533: Lewis W , et al. Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts. Proc. Natl. Acad. Sci. USA 93: 3592-3597, 1996. PubMed:
                32547: Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed:
                32564: Roesler WJ , et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed:
                32568: Lee JH , et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed:
                32723: Lieber A , et al. Recombinant adenoviruses with large deletions geneerated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J. Virol. 70: 8944-8960, 1996. PubMed:
                32752: Dubuisson J , Rice CM . Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. PubMed:
                32830: Yamaguchi Y , et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed:
                33015: Kounas MZ , et al. Cellular internalization and degradation of antithrombin III-thrombin, heparin cofactor II-thrombin, and alpha1-antitrypsin-trypsin complexes is mediated by the low density lipoprotein receptor-related protein. J. Biol. Chem. 271: 6523-6529, 1996. PubMed:
                33030: Klemm DJ , et al. Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms. J. Biol. Chem. 271: 8082-8088, 1996. PubMed:
                33038: Wu X , et al. Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of ApoB-containing lipoproteins. J. Biol. Chem. 271: 10277-10281, 1996. PubMed:
                33041: Ostlund RE Jr. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed:

                 

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