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                教育装备采购网首页 > 产品库 > 产品分类▃大全 > 实验室设备 > 实验室耗∞材 > 其它
                
        
                
            
                
                
                SW 1271 [SW-1271; SW1271]（人肺腺癌细胞）
                
                
                
                    
                
                        
                
                            
                SW 1271 [SW-1271; SW1271]（人肺腺癌细胞）
                
                            
                
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                                                    SW 1271 [SW-1271; SW1271]（人肺腺癌细胞）
                                                
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                ATCC
                
                            
                CRL-2177 (株)
                
                            
                上海锐聪科技发展有限公︽司
                
                            
                            
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                SW 1271 [SW-1271; SW1271]（人肺腺癌细胞）
                            
                                        
                SW 1271 [SW-1271; SW1271]（人肺腺癌细胞） 
                                            		        
                        
                                     
                        
                                        
                                                    
                                                                
                Cell Biology
                                                        		                    
                                    
                                        
                                            ATCC® Number:                            CRL-2177™                                 Price:                            $330.00                        
                                                        
                                                                
                Designations:
                                                        		                                                
                SW 1271 [SW-1271; SW1271]
                                                        		                                                
                Depositors:
                                                        		                                                
                W McCombs
                                                        		                    
                                    
                                                                
                Biosafety Level:
                                                        		                                                
                1
                                                        		                                                
                Shipped:
                                                        		                                                
                frozen
                                                        		                    
                                    
                                                                
                Medium & Serum:
                                                        		                                                
                See Propagation
                                                        		                                                
                Growth Properties:
                                                        		                                                
                adherent
                                                        		                    
                                    
                                                                
                Organism:
                                                        		                                                
                Homo sapiens (human)
                                                        		                                                
                Morphology:
                                                        		                                                
                epithelial
                                                        		                    
                                    
                                                             
                                    
                                        Source:                                                
                Organ: lung
                                        Disease: carcinoma; small cell lung cancer
                                                        		                    
                                    
                                        Permits/Forms:                                                
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                                                        		                    
                                    
                                                            
                                    
                                        Isolation:                                                
                Isolation date: 1976
                                        (The SW 1271 line was established in 1976 by A. Leibovitz from a grade II small cell carcinoma of the lung.)
                                                        		                    
                                    
                                        Antigen Expression:                                                
                Blood Type A; Rh +
                                                        		                    
                                    
                                        DNA Profile
                                        (STR):                		                                                
                Amelogenin: X
                                        CSF1PO: 11
                                        D13S317: 13
                                        D16S539: 11,12
                                        D5S818: 12
                                        D7S820: 9,12
                                        THO1: 7,8
                                        TPOX: 8
                                        vWA: 17,20
                                                        		                    
                                    
                                        Age:                                                
                69 years
                                                        		                    
                                    
                                        Gender:                                                
                male
                                                        		                    
                                    
                                        Ethnicity:                                                
                Caucasian
                                                        		                    
                                    
                                        Propagation:                                                
                ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                                        Temperature: 37.0C
                                        Atmosphere: air, 100%
                                                        		                    
                                    
                                        Subculturing:                                                
                Protocol:                        
                                              
                1. Remove and discard culture medium.
                  2.                             
                2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53% mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
                  3.                             
                3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 15 minutes).
                                              Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
                  4.                             
                4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
                  5.                             
                5. Add appropriate aliquots of cell suspension to new culture vessels.
                  6.                             
                6. Place culture vessels in incubators at 37C.
                  7.                         
                                        
                                        
                                        Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
                                        
                                        Medium renewal: 2 to 3 times per week
                                                        		                    
                                    
                                        Preservation:                                                
                Freeze medium: Culture medium, 95%; DMSO, 5%
                                        Storage temperature: liquid nitrogen vapor phase
                                                        		                    
                                                            
                                            		        
                    
                 
                
                
                
                
                
                
                    
                
                        
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