TOV-112D（人上皮◎性卵巢癌细胞）

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品　　牌：ATCC

产品型号：CRL-11731 (株)

制造厂商：上海锐聪科技发ω　展有限公司

所在地区：上海

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TOV-112D（人上皮性卵巢癌细胞）

TOV-112D（人上皮性卵巢癌细胞）

Cell Biology
ATCC^® Number:	CRL-11731™	Price:	$255.00
Designations:	TOV-112D	Depositors:	University of Montreal
Biosafety Level:	1	Shipped:	frozen
Medium & Serum:	See Propagation	Growth Properties:	adherent
Organism:	Homo sapiens (human)	Morphology:	epithelial

Source:	Organ: ovary Disease: primary malignant adenocarcinoma; endometrioid carcinoma Tumor stage: grade 3, stage IIIC
Cellular Products:	keratin [49408]
Permits/Forms:	In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
This material is cited in a U.S. and/or other Patent or Patent Application, and may not be used to infringe on the patent claims.
Related Cell Culture Products
Tumorigenic:	Yes, the cells are tumorigenic in nude mice and form colonies in soft agar
Oncogene:	her2/neu + [49408] p53 (mutated, Arg --> His mutation at exon 6, codon 175)
DNA Profile (STR):	Amelogenin: X CSF1PO: 12 D13S317: 8 D16S539: 9,12 D5S818: 10 D7S820: 9,10 THO1: 6 TPOX: 8,11 vWA: 18
Cytogenetic Analysis:	52, XX, add(X)(p22), +add(1)(p22), +add(1)(p22), +2, +9, +12, add(15)(p11), +17 [49408]
Age:	42 years adult
Gender:	female
Comments:	This cell line was initiated in October of 1992 from a patient with early onset ovarian cancer. The patient was of French-Canadian descent with an unknown family history of ovarian cancer. [49408] Unlike TOV-21G (ATCC CRL-11730) and OV-90 (ATCC CRL-11732), the TOV-112D cell line does not exhibit a deletion at chromosome 3p24. [42090]
Propagation:	ATCC complete growth medium: A 1:1 mixture of MCDB 105 medium and Medium 199, 85%; fetal bovine serum, 15% Temperature: 37.0C
Subculturing:	Protocol: Remove medium, add fresh 0.05% trypsin - 0.53 mM EDTA, rinse and remove trypsin. Allow the culture sit at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium renewal: Every 3 to 4 days
Preservation:	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature
Related Products:	recommended serum: ATCC 30-2020 purified DNA: ATCC CRL-11731
References:	42090: Mes-Masson AM , Provencher D . Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998 49408: Provencher DM , et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed:

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TOV-112D（人上皮性卵巢癌细胞）