| Designations: | Hs 578T | Depositors: | AJ Hackett | | Biosafety Level: | 1 | Shipped: | frozen | | Medium & Serum: | See Propagation | Growth Properties: | adherent | | Organism: | Homo sapiens (human) | Morphology: | epithelial  | | | | Source: | Organ: mammary gland; breast
Disease: carcinoma | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | Related Cell Culture Products | | Receptors: | estrogen receptor, not expressed [1119] | | Tumorigenic: | No, in immunosuppressed mice
Yes, in semisolid medium | DNA Profile
(STR): | Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,12
D5S818: 11
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 17 | Cytogenetic
Analysis: | Number of cells examined = 50; Modal Chromosome Number = 59 with a range of 50 to 77; Polyploidy Rate = 33.8%
Composite karyotype: 50-77 <3n> X, -1, del(1)(q12), -2, del(2)(?q36), der(3)t(3;15)(q10;p10),-4, -5, der(5)t(5;8)(p10;q10),-6, i(6)(p10), +8, -9, -10, -11, del(11)(p12), -12, -13, -14, -15, -15, -16, -17, -17, -17, i(17)(q10), -18, -19,der(19)(19pter<-q13::5q13<-qter), +22, +3 mar[cp12] | | Isoenzymes: | AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1; Me-2, 0; PGM1, 1; PGM3, 1 | | Age: | 74 years adult | | Gender: | female | | Ethnicity: | Caucasian | | Comments: | The Hs 578T cell strain was derived from a carcinoma of the breast.
It was originated by A.J. Hackett, et al. along with the Hs 578Bst (see ATCC HTB-125), which is a normal fibroblast-like line from the same patient.
The Hs 578T line had a mixed polygonal morphology initially, but a stellate cell type was selected for during passage and by cloning.
Aggregates of casein protein granules, desmosomes, tight junctions, lipid droplets and vesicularized smooth endoplasmic reticulum were observed by electron microscopy.
As with Hs 578Bst, no estrogen receptors or endogenous viruses were detected. | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.01 mg/ml bovine insulin; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% | | Subculturing: | Protocol: - Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Medium renewal: 2 to 3 times per week | | Preservation: | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase | | Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2002
recommended serum: ATCC 30-2020
normal (or near-normal) cell line established from the same patient: ATCC HTB-125
purified DNA: ATCC HTB-126D | | References: | 1119: Hackett AJ , et al. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs 578T) and the diploid myoepithelial (Hs 578Bst) cell lines. J. Natl. Cancer Inst. 58: 1795-1806, 1977. PubMed: 864756
22543: Smith HS . In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258
32275: Littlewood-Evans AJ , et al. The osteoclast-associated protease cathepsin K is expressed in human breast carcinoma. Cancer Res. 57: 5386-5390, 1997. PubMed: | |
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