Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

This line has a complex hyperdiploid karyotype of 66XX to 88XX.

female

The ES-2 cell line was established from a surgical tumor specimen taken from a 47 year old black woman.
The tumor was described as a poorly differentiated ovarian clear cell carcinoma.
Initially, the cells were grown in soft agar.
The cells exhibit low to moderate resistance to a number of chemotherapeutic agents including doxorubicin, cisplatin, carmustine, etoposide and cyanomorpholinodoxorubicin (MRA-CN).
ES-2 cells express low levels of P glycoprotein.

Protocol: Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium renewal: 2 to 3 times per week

24 hrs

23089: Lau DH , et al. Multifactorial mechanisms associated with broad cross-resistance of ovarian carcinoma cells selected by cyanomorpholino doxorubicin. Cancer Res. 51: 5181-5187, 1991. PubMed: