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                NCI-H1755 [H1755](人肺癌细◣胞)

                NCI-H1755 [H1755](人肺癌细〗胞)
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                • NCI-H1755 [H1755](人肺癌【细胞)
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                ATCC
                CRL-5892 (株)
                上海锐聪科技发展有限公司
                上海
                详细说明

                NCI-H1755 [H1755](人肺癌细胞)

                NCI-H1755 [H1755](人肺癌细胞)
                 
                Cell Biology
                ATCC® Number: CRL-5892™ Price: $330.00
                Designations:
                NCI-H1755 [H1755]
                Depositors:
                AF Gazdar
                JD Minna
                1
                Shipped:
                frozen
                Medium & Serum:
                Growth Properties:
                mixed, adherent and suspension
                Organism:
                Homo sapiens (human)
                Morphology:
                Epithelial
                 
                Source:
                Organ: lung
                Disease: adenocarcinoma; non-small cell lung cancer
                Tumor stage: stage 4
                Derived from metastatic site: liver
                Permits/Forms:
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                Restrictions:
                The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056, FAX (214) 688-7233.
                DNA Profile
                (STR):
                Amelogenin: X
                CSF1PO: 12
                D13S317: 10,12
                D16S539: 11,12
                D5S818: 11,12
                D7S820: 12
                F13A01: 4,6
                F13B: 8,10
                FESFPS: 11
                LPL: 12
                THO1: 7
                TPOX: 8
                vWA: 18
                Age:
                65 years
                Gender:
                female
                Ethnicity:
                Caucasian
                Comments:
                The line was established in October 1987.
                The patient was a smoker.
                60 pack years.
                Propagation:
                ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
                Temperature: 37.0C
                Subculturing:
                Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium.Cultures grow as floating aggregates of round cell clusters and attached epithelial-like cells. Attached cells can be subcultured using 0.25% Trypsin-0.53 mM EDTA.
                Preservation:
                Culture medium, 95%; DMSO, 5%
                Related Products:
                Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2001
                recommended serum: ATCC 30-2020
                References:
                23570: NCI-Navy Medical Oncology Branch Cell Line Supplement. J. Cell. Biochem. suppl. 24: 1996.

                 

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