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                A-704(人肾癌细胞)

                A-704(人肾癌细胞)
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                • A-704(人肾癌细胞)
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                ATCC
                HTB-45 (株)
                上海锐聪科技发展有限公司
                上海
                详细说明

                A-704(人肾癌细胞)

                A-704(人肾癌细胞)
                 
                Cell Biology
                ATCC® Number: HTB-45™ Price: $330.00
                Designations:
                A-704
                Depositors:
                DJ Giard
                1
                Shipped:
                frozen
                Medium & Serum:
                Growth Properties:
                adherent
                Organism:
                Homo sapiens (human)
                Morphology:
                epithelial
                 
                Source:
                Organ: kidney
                Disease: adenocarcinoma
                Permits/Forms:
                In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
                Applications:
                Tumorigenic:
                No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
                DNA Profile
                (STR):
                Amelogenin: X,Y
                CSF1PO: 7,8
                D13S317: 8
                D16S539: 12,13
                D5S818: 10,11
                D7S820: 10
                THO1: 7,9
                TPOX: 11
                vWA: 14,18
                Cytogenetic
                Analysis:
                (P59) diploid to hyperdiploid, hypertriploid to hypertetraploid with abnormalities including breaks, dicentrics and endoreduplication
                Isoenzymes:
                AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 2; Me-2, 1; PGM1, 1; PGM3, 1-2
                Age:
                78 years
                Gender:
                male
                Comments:
                The A-704 line was derived by D.J. Giard as indicated in the description for ATCC HTB-41.
                Propagation:
                ATCC complete growth medium: Minimum essential medium (Eagle) in Earles BSS with nonessential amino acids and sodium pyruvate, 85%; fetal bovine serum, 15%
                Subculturing:
                Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
                Add fresh medium, aspirate and dispense into new flasks.
                Subculture every 6 to 8 days.
                Subcultivation ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

                Medium renewal: 2 to 3 times per week
                Preservation:
                Culture medium, 95%; DMSO, 5%
                References:
                23218: Giard DJ , et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed:
                24381: Fogh J . Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

                 

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